Exosome (Cell Media) ELISA Kit (Chemiluminescence)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-60740
    • Availability
      Product Discontinued

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Exosome (Cell Media) ELISA Kit (Chemiluminescence) Summary

Sensitivity
39 pg protein equivalent
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 13%
Intra-Assay
CV% < 10%
Sample Volume
100 ul
Kit Type
ELISA Kit (Chemiluminescence)

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. Overall exosome immunocapture and quantification from cell culture supernatant.

Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Exosome Standards
  2. HRP conjugated secondary antibody
  3. Immunoplate (White)
  4. Primary antibody
  5. Sample buffer (1X)
  6. Substrate luminometric Solution A
  7. Substrate luminometric Solution B
  8. Washing buffer (25X)

Alternate Names for Exosome (Cell Media) ELISA Kit (Chemiluminescence)

  • EV detection
  • exosome detection
  • extracellular vesicle detection

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Exosome (Cell Media) ELISA Kit (NBP2-60740). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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