Exosome (Cell Media) ELISA Kit (Chemiluminescence) Summary
| Sensitivity |
39 pg protein equivalent |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 13% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Chemiluminescence) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. Overall exosome immunocapture and quantification from cell culture supernatant.
Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively). |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Exosome Standards
- HRP conjugated secondary antibody
- Immunoplate (White)
- Primary antibody
- Sample buffer (1X)
- Substrate luminometric Solution A
- Substrate luminometric Solution B
- Washing buffer (25X)
|
Alternate Names for Exosome (Cell Media) ELISA Kit (Chemiluminescence)
Background
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for Exosome (Cell Media) ELISA Kit (NBP2-60740) (0)
There are no publications for Exosome (Cell Media) ELISA Kit (NBP2-60740).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Exosome (Cell Media) ELISA Kit (NBP2-60740) (0)
There are no reviews for Exosome (Cell Media) ELISA Kit (NBP2-60740).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Exosome (Cell Media) ELISA Kit (NBP2-60740). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
Additional Exosome (Cell Media) Products
Blogs on Exosome (Cell Media)