Exosome (Cell Media) ELISA Kit (Chemiluminescence)

Product Discontinued

Exosome (Cell Media) ELISA Kit (Chemiluminescence) Summary

• Sensitivity: 39 pg protein equivalent
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 13%
• Intra-Assay: CV% < 10%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Chemiluminescence)

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. Overall exosome immunocapture and quantification from cell culture supernatant.
  
  Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Exosome (Cell Media) ELISA Kit (Chemiluminescence)

• EV detection
• exosome detection
• extracellular vesicle detection

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Exosome (Cell Media) ELISA Kit (NBP2-60740). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.