Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit Summary
| Description |
Fast and user friendly protocol Small starting amount of sample Better RNA yield than similar products Exosome Standards included as control RNA extraction from overall exosome population captured with immunobeads
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab287871) |
| Kit Type |
Extraction Kit |
Applications/Dilutions
| Application Notes |
Direct capture and exosome RNA extraction from human biofluids and cell culture media without initial exosome purification step
Starting from 0.1 ml of biological sample (plasma) per reaction. Whole plasma and serum can be directly used for capture. Concentrated urine and cell culture supernatant samples are recommended prior capture according to our suggested protocol, Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray). |
Reactivity Notes
Exosomal RNA isolation from human biological fluids including plasma, serum, urine and cell culture media.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- 1.5 ml RNase free elution tubes
- 5X Bead Washing buffer
- Columns
- Elution buffer
- Lyophilized Exosome Standard
- Lysis buffer
- Pre-couple latex immunobeads
- RNA Washing buffer (to add Ethanol 96%)
|
Alternate Names for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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