Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit

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Overall Exosome Capture and RNA Extraction (Biofluids, Cell Media) Kit [NBP2-49784] - Expected Nanodrop profile for RNA extracted from immunocaptured exosomes (100 ul of human plasma). Yield = 8.4 ng/ul; A260/280 =1.6; ...read more
Overall Exosome Capture and RNA Extraction (Biofluids, Cell Media) Kit [NBP2-49784] - Comparasion of total RNA yield (Nanodrop quantification) for exosomal RNA extraction kits from Novus Biologicals' and a competitor

Order Details


    • Catalog Number
      NBP2-49784
    • Availability
      Product Discontinued

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Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit Summary

Description
Fast and user friendly protocol
Small starting amount of sample
Better RNA yield than similar products
Exosome Standards included as control
RNA extraction from overall exosome population captured with immunobeads

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab287871)
Kit Type
Extraction Kit

Applications/Dilutions

Application Notes
Direct capture and exosome RNA extraction from human biofluids and cell culture media without initial exosome purification step

Starting from 0.1 ml of biological sample (plasma) per reaction. Whole plasma and serum can be directly used for capture. Concentrated urine and cell culture supernatant samples are recommended prior capture according to our suggested protocol, Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray).

Reactivity Notes

Exosomal RNA isolation from human biological fluids including plasma, serum, urine and cell culture media.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.5 ml RNase free elution tubes
  2. 5X Bead Washing buffer
  3. Columns
  4. Elution buffer
  5. Lyophilized Exosome Standard
  6. Lysis buffer
  7. Pre-couple latex immunobeads
  8. RNA Washing buffer (to add Ethanol 96%)

Alternate Names for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit

  • exosome capture and quantification

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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FAQs for Exosome Capture (Biofluids, Cell Media) and RNA Kit (NBP2-49784). (Showing 1 - 1 of 1 FAQ).

  1. Does this product work for saliva?
    • This product NBP2-49784 should work for saliva as well as other bio-fluids.  The plates are all the same really and target the exosomes and it does not matter what the source of the exosome is. We do not have a quantitative comparison for the amount of exosomes/RNA recovered in the different bio-fluids.

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