Recombinant Exendin-4 Protein

SDS-Page: Recombinant Reptile Exendin-4 Protein [NBP2-34945] - Exendin-4 Protein [NBP2-34945]

• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Exendin-4 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 39 amino acids corresponding to Exendin-4 Source: E. coli

  Uniprot ID: P26349

  Amino Acid Sequence: HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS

• Details of Functionality: 1. Regulates Glucose levels rapidly;
  2. Reduces Insulin resistance;
  3. Reduces Glucagon;
  4. Reduces HbA1c;
  5. Stimulates beta cell growth which stimulates insulin production.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 10 EU/mg of Exendin-4 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 4.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Exendin-4 Protein

• Exendin4

Background

Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53 % sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucosedependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes beta-cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA sequence encoding the 39 amino acid of Exendin-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.