EV Precipitation Solution (Cell Culture)

• Suitable Sample Type: Cell culture supernatant

EV Precipitation Solution (Cell Culture) Summary

• Description:

  EV Precipitation Solution (Cell Culture) is a simple and rapid method of high yield extracellular vesicle (EV) isolation from urine samples. Based on ploymer precipitation technique, samples are incubated with EV Precipitation Solution on ice and exosomes are isolated after a gentle table-top centrifugation step. Advantages of EV Precipitation Solution include: High yield of EVs, No prior sample concentration required, No special equipment. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.

• Sample Volume: 1-5 mL

Applications/Dilutions

• Application Notes: A 25mL solution is equalivant to 25 reactions (cell culture media)

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Alternate Names for EV Precipitation Solution (Cell Culture)

• EV isolation
• exosome isolation
• extracellular vesicle isolation

Background

For precipitation techniques to isolate extracellular vesicles, samples containing EVs are incubated with a buffer that changes their solubility or sedimentation rate. One common precipitation buffer uses polyethyleneglycol (PEG), a hydrophilic polymer. By incubating EVs with PEG, it decreases the solubility of EVs in solution and, using a very gentle centrifugation step, causes accumulation of EVs at the bottom of the tube.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.