EV Precipitation Solution (Blood)

EV Precipitation Solution (Blood) [NBP3-11765] - Plasma - Exosomes isolated from plasma were resuspended in 100 ul of PBS and protein contents quantified by BCA assay. 60 ug of total protein contents were loaded with Laemmli Sample buffer 5X on acrylamide gel. WB performed using anti-ALIX antibody. Ultracentrifuged exosomes used as control (UC) Serum - Exosomes isolated from serum were resuspended in 24 ul of PBS 1X, and the entire amount was loaded with 6 ul of Laemmli Sample buffer 5X on acrylamide gel.

EV Precipitation Solution (Blood) [NBP3-11765] - Plasma and serum: After exosome isolation resuspend pellet in 100 ul of PBS 1X and load the entire amount in a well of an ELISA plate. Example reports CD9 expression in isolated exosomes from different volumes of plasma.

EV Precipitation Solution (Blood) [NBP3-11765] - Exosome pellet can be directly lysed with lysis buffers for nucleic acids extraction. If RNA extraction is performed by using Trizol or similar organic reagents we suggest resuspending exosomes in PBS, then to add Trizol into the mixture and to proceed with RNA purification.

• Suitable Sample Type: Plasma and serum

EV Precipitation Solution (Blood) Summary

• Description:

  EV Precipitation Solution (Blood) is a simple and rapid method of high yield extracellular vesicle (EV) isolation from biofluids such as serum and plasma. Based on ploymer precipitation technique, samples are incubated with EV Precipitation Solution on ice and exosomes are isolated after a gentle table-top centrifugation step. EV isolation with EV Precipitation Solution is fast and requires no special equipment. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.

• Sample Volume: 100-250 uL (plasma) or 250-500 uL (serum)

Applications/Dilutions

• Application Notes: A 5mL solution is equivalent to 180 reactions (plasma) and 80 reactions (serum).

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Alternate Names for EV Precipitation Solution (Blood)

• EV isolation
• exosome isolation
• extracellular vesicle isolation

Background

For precipitation techniques to isolate extracellular vesicles, samples containing EVs are incubated with a buffer that changes their solubility or sedimentation rate. One common precipitation buffer uses polyethyleneglycol (PEG), a hydrophilic polymer. By incubating EVs with PEG, it decreases the solubility of EVs in solution and, using a very gentle centrifugation step, causes accumulation of EVs at the bottom of the tube.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.