EV MiniSEC Columns

EV MiniSEC Columns [NBP3-41043] - EV separation - EV MiniSEC Column was filled with 500 uL of medium from HCT116 cells, previously concentrated by TFF-Easy. 20 fractions (100 μl each one) have been collected and analyzed by ELISA assay (CD81 marker) and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 9 - 14 (turnaround time approximately 10 min), whereas circulating proteins corresponded to the fractions 16 - 20.

• Suitable Sample Type: Biofluids or cell culture supernatant

EV MiniSEC Columns Summary

• Description:

  PURE-EVs are size exclusion chromatography (SEC) columns designed to isolate extracellular vesicles (EVs) from small volume samples. Compared to other techniques, PURE-EV efficiently separates EVs from circulating proteins while maintaining original shape and functionality of vesicles. Highly pure EV preparations, no residual chemicals from precipitation, and rapid isolation in approximately 20 minutes. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.

• Sample Volume: 100 uL - 500 uL

Packaging, Storage & Formulations

• Storage: Store at 4C.

Background

Size-exclusion chromatography (SEC) separates macromolecules based on their size. To accomplish this, the sample is passed through a porous column and molecules are isolated in fractions from the eluate. The size of the pores can vary depending on the size of the desired product. For isolation of EVs from proteins and lipoproteins, pore sizes can vary from ~40-80 nm. As the sample passes through the column, molecules smaller than the pore size will get trapped in the pores, slowing them down, while larger molecules will pass through. This results in the larger molecules, such as EVs, eluting from the column in earlier fractions, while smaller molecules, such as proteins and protein aggregates, are eluted in later fractions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.