Human Estradiol ELISA Kit (Colorimetric)

ELISA: Estradiol ELISA Kit [HRP] [KA0234] - Standard curve

Product Discontinued

Human Estradiol ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. Estradiol (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of estradiol.
• Standard Curve Range: 10 to 1000 pg/mL
• Sensitivity: 10 pg/mL
• Assay Type: competitive ELISA
• Inter-Assay: CV% < 12.50%
• Intra-Assay: CV% < 10.85%
• Spike Recovery: 101.30%
• Sample Volume: 25 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Estradiol ELISA Kit (Colorimetric)

• 17-Beta-Estradiol;17-beta-HSD
• Estradiol
• Estrogen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Estradiol ELISA Kit (KA0234)(4)

Publications using KA0234

• O'Leary CB, Lehman C, Koltun K et al. Response of testosterone to prolonged aerobic exercise during different phases of the menstrual cycle. Eur J Appl Physiol. 2013-06-28 [PMID: 23812088]
• Greising SM, Carey RS, Blackford JE et al. Estradiol treatment, physical activity, and muscle function in ovarian-senescent mice. Exp Gerontol. 2011-05-04 [PMID: 21570459]
• Greising SM, Baltgalvis KA, Kosir AM et al. Estradiol's beneficial effect on murine muscle function is independent of muscle activity. J Appl Physiol. 2010-10-21 [PMID: 20966194]
• Baltgalvis KA, Greising SM, Warren GL, Lowe DA. Estrogen regulates estrogen receptors and antioxidant gene expression in mouse skeletal muscle. PLoS One. 2010-04-13 [PMID: 20405008]

FAQs for Estradiol ELISA Kit (KA0234). (Showing 1 - 2 of 2 FAQs).

1. I'm wondering how many sample I can analyse with 1 kit KA1907
   • This ELISA kit comes with a 96 well plate. After using 12 wells for the standard and duplication, 84 wells are left. If you are testing your samples in duplicate, 84/2 = 42 samples can be analized. If testing in triplicate, 84/3 = 28 samples.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.