| Description | Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue. Whole cell lysate (non-denatured) |
| Localization | Embryonic |
| Preparation Method |
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | In modified RIPA Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF). |
| Concentration | 4 mg/ml |
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