ER-HR3 Antibody (ER-HR3) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clone: ER-HR3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

ER-HR3 Antibody (ER-HR3) [Alexa Fluor® 488] Summary

• Immunogen: Adherent F1 (CBAxBL) bone marrow stormal cells.
• Specificity: NB100-64931 recognizes the murine antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in haematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low. During foetal development, ER-HR3 positive cells are localised to haemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response. Clone ER-HR3 does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies. Clone ER-HR3 recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ER-HR3 Antibody (ER-HR3) [Alexa Fluor® 488]

• Hematopoiesis related macrophage

Background

Macrophage are any of the many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, circulating for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content and become macrophages. The morphology of macrophages varies among different tissues and between normal and pathologic states, and not all macrophages can be identified by morphology alone. However, most macrophages are large cells with a round or indented nucleus, a well developed Golgi apparatus, abundant endocytotic vacuoles, lysosomes, and phagolysosomes, and a plasma membrane covered with ruffles or microvilli. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes (lysozyme, collagenases, elastase, acid hydrolases), several complement components and coagulation factors, some prostaglandins and leukotrienes, and several regulatory molecules (interferon, interleukin 1). Among the cells now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans type and foreign body type multinucleated giant cells (in inflamed tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.