ER-HR3 Antibody (ER-HR3)

Images

 
There are currently no images for ER-HR3 Antibody (NB100-64931).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
ER-HR3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ER-HR3 Antibody (ER-HR3) Summary

Immunogen
Adherent F1 (CBAxBL) bone marrow stormal cells.
Specificity
NB100-64931 recognizes the murine antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in haematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low. During foetal development, ER-HR3 positive cells are localised to haemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response. Clone ER-HR3 does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies. Clone ER-HR3 recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.
Isotype
IgG2c
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ER-HR3 Antibody (ER-HR3)

  • Hematopoiesis related macrophage

Background

Macrophage are any of the many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, circulating for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content and become macrophages. The morphology of macrophages varies among different tissues and between normal and pathologic states, and not all macrophages can be identified by morphology alone. However, most macrophages are large cells with a round or indented nucleus, a well developed Golgi apparatus, abundant endocytotic vacuoles, lysosomes, and phagolysosomes, and a plasma membrane covered with ruffles or microvilli. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes (lysozyme, collagenases, elastase, acid hydrolases), several complement components and coagulation factors, some prostaglandins and leukotrienes, and several regulatory molecules (interferon, interleukin 1). Among the cells now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans type and foreign body type multinucleated giant cells (in inflamed tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER-HR3 Antibody (NB100-64931) (0)

There are no publications for ER-HR3 Antibody (NB100-64931).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ER-HR3 Antibody (NB100-64931) (0)

There are no reviews for ER-HR3 Antibody (NB100-64931). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for ER-HR3 Antibody (NB100-64931) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64931AF405
Alexa Fluor 488 Labeled NB100-64931AF488
Alexa Fluor 647 Labeled NB100-64931AF647
Alexa Fluor 700 Labeled NB100-64931AF700
Allophycocyanin Labeled NB100-64931APC
Biotin Labeled NB100-64931B
DyLight 350 Labeled NB100-64931UV
DyLight 405 Labeled NB100-64931V
DyLight 488 Labeled NB100-64931G
DyLight 680 Labeled NB100-64931FR
DyLight 755 Labeled NB100-64931IR
PE Labeled NB100-64931PE
PerCP Labeled NB100-64931PCP

Additional ER-HR3 Products

ER-HR3 NB100-64931

Blogs on ER-HR3

There are no specific blogs for ER-HR3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ER-HR3 Antibody (ER-HR3) and receive a gift card or discount.