Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

Epinephrine/Norepinephrine ELISA Kit [KA1877] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

• Applications: ELISA, NULL
• Suitable Sample Type: Plasma, Urine
• Standard Curve Range: Adrenaline: 0.7 to 200 ng/mL in Urine; 18 to 6667 pg/mL in Plasma, Noradrenaline: 2.5 to 1000 ng/mL in Urine; 93 to 33333 pg/mL in Plasma
• Sensitivity: Adrenaline: 0.9 ng/mL Urine, 10 pg/mL Plasma
  Noradrenaline: 1.7 ng/mL Urine, 36 pg/mL Plasma

Epinephrine/Norepinephrine ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. Epinephrine/Norepinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Epinephrine and Norepinephrine in plasma and urine. This product contains two assay plates, for Epinephrine and Normetanephrine separately.
• Standard Curve Range: Adrenaline: 0.7 to 200 ng/mL in Urine; 18 to 6667 pg/mL in Plasma, Noradrenaline: 2.5 to 1000 ng/mL in Urine; 93 to 33333 pg/mL in Plasma
• Sensitivity: Adrenaline: 0.9 ng/mL Urine, 10 pg/mL Plasma
  Noradrenaline: 1.7 ng/mL Urine, 36 pg/mL Plasma
• Assay Type: Quantification
• Inter-Assay: Epinephrine: 15.6%, Norepinephrine: 11.75%
• Intra-Assay: Epinephrine: 12.57%, Norepinephrine: 12.95%
• Spike Recovery: Epinephrine: 107% in Urine, 92% in Plasma Norepinephrine: 109% in Urine, 97% in Plasma
• Sample Volume: Adrenaline ELISA : 100 uL (extracted)
  Noradrenaline ELISA : 20 uL (extracted)
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

• adrenaline
• Arterenol
• l-Adrenaline
• L-epinephrine
• Levarterenol
• Levoepinephrine
• L-Noradrenaline
• L-Norepinephrine
• noradrenaline

Background

Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Epinephrine/Norepinephrine ELISA Kit (KA1877)(54)

Publications using KA1877

• Francisco C, Daniela S, Sofia T et al. The Secretome of Parental and Bone Metastatic Breast Cancer Elicits Distinct Effects in Human Osteoclast Activity after Activation of ?2 Adrenergic Signaling. Biomolecules. 2023-03-30 [PMID: 37189370]
• William D, Christopher C, Katherine S et al. A method for measuring meaningful physiological variables in fish blood without surgical cannulation. Sci Rep. 2023-01-17 [PMID: 36650204]
• Aditya N, Phaneendra B, Brou K et al. RNA-Seq exploration of the influence of stress on meat quality in Spanish goats. Sci Rep. 2022-11-29 [PMID: 36446782]
• Fang Y, Raphael C, Asha E et al. Chronic reduction of store operated Ca2+ entry is viable therapeutically but is associated with cardiovascular complications. J Physiol. 2022-10-01 [PMID: 36181482]
• Porter E, Clow K, Sandrelli R et al. Acute and chronic cold exposure differentially affect cardiac control, but not cardiorespiratory function, in resting Atlantic salmon (S almo salar). Curr Res Physiol. 2022-03-17 [PMID: 35359619]
• Cheng-Hu H, Bing-Dong S, Jin L et al. Sympathetic Neurostress Drives Osteoblastic Exosomal MiR-21 Transfer to Disrupt Bone Homeostasis and Promote Osteopenia. Small Methods. 2021-12-16 [PMID: 35312228]
• Yan-Ting C, Qi-Yuan Y, Yun H et al. Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. Nat Commun. 2021-11-25 [PMID: 34824246]
• Chaobao L, Yang Y, Cheng C et al. Environmental eustress modulates ?-ARs/CCL2 axis to induce anti-tumor immunity and sensitize immunotherapy against liver cancer in mice. Nat Commun. 2021-09-30 [PMID: 34593796]
• Alison L, Michael S, Emily K et al. Inflammation and metabolism gene sets in subcutaneous abdominal adipose tissue are altered 1 hour after exercise in adults with obesity. J Appl Physiol (1985). 2021-08-19 [PMID: 34410849]
• Marcel W, Calum W, Giulia V et al. Kefir ameliorates specific microbiota-gut-brain axis impairments in a mouse model relevant to autism spectrum disorder. Brain Behav Immun. 2021-07-09 [PMID: 34252569]
• Govind K, Zaira E, Phaneendra B et al. Social isolation of goats: significance of visual contact with conspecifics on behavioral and physiological responses. J Anim Sci. 2021-06-01 [PMID: 33959763]
• Charlotte D, Hsu-Wen T, Malha C et al. Local and systemic factors drive ectopic osteogenesis in regenerating muscles of spinal cord-injured mice in a lesion level-dependent manner. J Neurotrauma. 2021-06-18 [PMID: 33913747]
• Guo Y, Xiaokereti J, Meng Q et al. Low-Level Vagus Nerve Stimulation Reverses Obstructive Sleep Apnea-Related Atrial Fibrillation by Ameliorating Sympathetic Hyperactivity and Atrial Myocyte Injury Frontiers in Physiology 2021-01-26 [PMID: 33574766]
• Hashim I, Alessandra A, Jacob B et al. Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle. Pflugers Arch. 2021-01-08 [PMID: 33420549]
• HJ Wang, CS Lee, RSZ Yee, L Groom, I Friedman, L Babcock, DK Georgiou, J Hong, AD Hanna, J Recio, JM Choi, T Chang, NH Agha, J Romero, P Sarkar, N Voermans, MW Gaber, SY Jung, ML Baker, RG Pautler, RT Dirksen, S Riazi, SL Hamilton Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation Nat Commun, 2020-10-09;11(1):5099. 2020-10-09 [PMID: 33037202]
• Natalia T, Eszter K, Krisztina F et al. Pyrophosphate therapy prevents trauma-induced calcification in the mouse model of neurogenic heterotopic ossification. J Cell Mol Med. 2020-09-04 [PMID: 32885586]
• Dyana S, Isabella L, Emanoel O et al. Cardiovascular Protection Variables Based on Exercise Intensity in Stable Coronary Heart Disease Patients After Coronary Stenting: A Comparative Study. Vasc Health Risk Manag. 2020-07-06 [PMID: 32753874]
• Maghawry H, Mostafa E, Ahmed A et al. Pioglitazone ameliorates high fat diet-induced hypertension and induces catechol o-methyl transferase expression in rats. Eur J Pharmacol. 2020-08-01 [PMID: 32750363]
• Dokyoung Y, Hunter H, Thomas W et al. Hyperalgesia After a Drinking Episode in Young Adult Binge Drinkers: A Cross-Sectional Study. Alcohol Alcohol. 2020-06-01 [PMID: 32476005]
• Xu Z, Bo L, Yuan Y et al. Brain Control of Humoral Immune Responses Amenable to Behavioural Modulation. Nature. 2020-04-29 [PMID: 32405000]
• Jayalakshmi L, Yang G, Chunyan H et al. Nephron-Specific Disruption of Polycystin-1 Induces Cyclooxygenase-2-Mediated Blood Pressure Reduction Independent of Cystogenesis. J Am Soc Nephrol. 2020-04-16 [PMID: 32300065]
• van M, Lyte J, Boehme M et al. The Role of the Microbiota in Acute Stress-Induced Myeloid Immune Cell Trafficking. Brain Behav Immun. 2019-12-05 [PMID: 31812778]
• Nicholas P, Hashim I, Patrick D et al. A novel gravity-induced blood flow restriction model augments ACC phosphorylation and PGC-1? mRNA in human skeletal muscle following aerobic exercise: A randomized crossover study. Appl Physiol Nutr Metab. 2019-11-28 [PMID: 31778310]
• Zhang Y, Chi X, Wang Z et al. Protective effects of Panax notoginseng saponins on PME-Induced nephrotoxicity in mice. Biomed Pharmacother. 2019-05-16 [PMID: 31103823]
• Sudo H, Tsuji AB, Sugyo A et al. Preclinical Evaluation of the Acute Radiotoxicity of the alpha -Emitting Molecular-Targeted Therapeutic Agent 211At-MABG for the Treatment of Malignant Pheochromocytoma in Normal Mice. Transl Oncol. 2019-05-08 [PMID: 31078058]
• SJ Roy, BG Fico, BD Baker, SS Lapierre, JA Shah, DD Gourley, LA Delfausse, H Tanaka Effects of full-fat dairy products on subclinical vascular function in adults with elevated blood pressure: a randomized clinical trial Eur J Clin Nutr, 2019-04-25;0(0):. 2019-04-25 [PMID: 31024092]
• Rao X, Asico LD, Zanos P et al. Alpha2B-Adrenergic Receptor Overexpression in the Brain Potentiate Air Pollution-induced Behavior and Blood Pressure Changes. Toxicol Sci. 2019-05-01 [PMID: 30812033]
• Kliszczewicz B, Bechke E, Williamson C et al. Citrus Aurantium and caffeine complex versus placebo on biomarkers of metabolism: a double blind crossover design. J Int Soc Sports Nutr. 2019-02-06 [PMID: 30728061]
• Kim JA, Ahn HJ, Yang M et al. Intraoperative use of dexmedetomidine for the prevention of emergence agitation and postoperative delirium in thoracic surgery: a randomized-controlled trial. Can J Anaesth. 2019-01-24 [PMID: 30710258]
• Bonavia A, Verbeek T, Adhikary S et al. A randomized controlled trial comparing methohexital and propofol for induction in patients receiving angiotensin axis blockade. Medicine (Baltimore). 2019-02-15 [PMID: 30702630]
• Hashimoto O, Funaba M, Sekiyama K et al. Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine. Cell Rep. 2018-10-30 [PMID: 30380411]
• Ribeiro-da-Silva M, Vasconcelos DM, Alencastre IS et al. Interplay between sympathetic nervous system and inflammation in aseptic loosening of hip joint replacement. Sci Rep. 2018-10-30 [PMID: 30375409]
• Boehme J, Le Moan N, Kameny RJ et al. Preservation of myocardial contractility during acute hypoxia with OMX-CV, a novel oxygen delivery biotherapeutic. PLoS Biol. 2018-10-18 [PMID: 30335746]
• Kirino K, Nakahata T, Taguchi T et al. Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition. Sci Rep 2018-08-27 [PMID: 30150715]
• Vasamsetti SB, Florentin J, Coppin E et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity 2018-06-26 [PMID: 29958804]
• Loftus TJ, Mira JC, Miller ES et al. The Post-injury Inflammatory State and the Bone Marrow Response to Anemia. Am J Respir Crit Care Med 2018-05-16 [PMID: 29768025]
• Xi P, Du J, Liang H et al. Intraventricular Injection of LKB1 Inhibits the Formation of Diet-Induced Obesity in Rats by Activating the AMPK-POMC Neurons-Sympathetic Nervous System Axis Cell. Physiol. Biochem. 2018-05-09 [PMID: 29763913] (ELISA)
• Jackson DA, Michael T, Vieira de Abreu A et al. Prevention of Severe Hypoglycemia-Induced Brain Damage and Cognitive Impairment with Verapamil. Diabetes 2018-05-03 [PMID: 29724724]
• Macias D, Cowburn A. S, et al. HIF-2 alpha is essential for carotid body development and function. Elife 2018-04-19 [PMID: 29671738]
• Dhar P, Sharma VK, Das SK et al. Differential responses of autonomic function in sea level residents, acclimatized lowlanders at >3500 m and Himalayan high altitude natives at >3500 m: A cross-sectional study. Respir Physiol Neurobiol 2018-04-09 [PMID: 29649580]
• Kushnir A, Santulli G, Reiken SR et al. Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure. Circulation 2018-03-28 [PMID: 29593014]
• Zhu RT, Liu XH, Shi YW et al. Propranolol can induce PTSD-like memory impairments in rats. Brain Behav 2018-01-18 [PMID: 29484264]
• Perry RJ, Wang Y, Cline GW et al. Leptin Mediates a Glucose-Fatty Acid Cycle to Maintain Glucose Homeostasis in Starvation. Cell 2018-01-04 [PMID: 29307489]
• Hu B, Zhang J, Wang J et al. Responses of PKCepsilon to cardiac overloads on myocardial sympathetic innervation and NET expression. Auton Neurosci 2018-03-01 [PMID: 29195789] (Rat)
• Bubak MP, Heesch MWS, Shute RJ et al. Irisin and Fibronectin Type III Domain-Containing 5 Responses to Exercise in Different Environmental Conditions. Int J Exerc Sci 2017-09-01 [PMID: 28966707]
• Samad MB, Mohsin MNAB, Razu BA et al. [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic B-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemi BMC Complement Altern Med 2017-08-09 [PMID: 28793909]
• Zhang LC, Huang Z, Li PB et al. Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage. Exp Ther Med 2017-05-05 [PMID: 28587398]
• Perry RJ, Peng L, Abulizi A et al. Mechanism for leptin's acute insulin-independent effect to reverse diabetic ketoacidosis. J Clin Invest 2017-02-01 [PMID: 28112679]
• Sinclair M, Keeley T, Lefebvre AC, Phillips CJ Behavioral and Physiological Responses of Calves to Marshalling and Roping in a Simulated Rodeo Event. Animals (Basel) 2016-04-28 [PMID: 27136590]
• White CW, Lillico R, Sandha J et al. Physiologic Changes in the Heart Following Cessation of Mechanical Ventilation in a Porcine Model of Donation After Circulatory Death: Implications for Cardiac Transplantation. Am J Transplant 2016-03-01 [PMID: 26663659]
• Brain KL, Allison BJ, Niu Y et al. Induction of controlled hypoxic pregnancy in large mammalian species. Physiol Rep 2015-12-01 [PMID: 26660546] (ELISA)
• Tsuda H, Kotani T, Sumigama S et al. Impact of warning bleeding on the cortisol level in the fetus and neonatal RDS/TTN in cases of placenta previa. J Matern Fetal Neonatal Med 2015-06-01 [PMID: 25001429]
• Kwon BS, Ha CM, Yu S et al. Microscopic nodes and ducts inside lymphatics and on the surface of internal organs are rich in granulocytes and secretory granules. Cytokine. 2012-08-09 [PMID: 22884518]
• Wang Y, Nasiri AR, Damsky WE et al. Uncoupling Hepatic Oxidative Phosphorylation Reduces Tumor Growth in Two Murine Models of Colon Cancer. Cell Rep 2018-07-03 [PMID: 9972790]

FAQs for Epinephrine/Norepinephrine ELISA Kit (KA1877). (Showing 1 - 5 of 5 FAQs).

1. Can it be used with the samples from sheep?
   • The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
2. Can it be used with tissue homogenates?
   • This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
3. Is this assay for use in rat plasma? The species is not listed.
   • The kit should work for any species as the target should be the same across all species.
4. Can this kit be used with rat serum/plasma?
   • Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
5. Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
   • This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.