Epinephrine ELISA Kit (Colorimetric)

ELISA: Epinephrine ELISA Kit [KA1882] - Standard curve

• Applications: ELISA, NULL
• Suitable Sample Type: Plasma, Urine
• Standard Curve Range: 0.7 to 200 ng/mL in Urine, 18 to 6667 pg/mL in plasma
• Sensitivity: 0.9 ng/mL urine, 10 pg/mL plasma

Epinephrine ELISA Kit (Colorimetric) Summary

• Description: Epinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Epinephrine in plasma and urine.
• Standard Curve Range: 0.7 to 200 ng/mL in Urine, 18 to 6667 pg/mL in plasma
• Sensitivity: 0.9 ng/mL urine, 10 pg/mL plasma
• Assay Type: Quantification
• Inter-Assay: CV% < 10.27%
• Intra-Assay: CV% < 10.95%
• Sample Volume: 100 uL (extracted)
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Epinephrine ELISA Kit (Colorimetric)

• adrenaline
• l-Adrenaline
• L-epinephrine
• Levoepinephrine

Background

Adrenaline (epinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Epinephrine ELISA Kit (KA1882)(10)

Publications using KA1882

• X Jia, S Chen, X Li, S Tao, J Lai, H Liu, K Huang, Y Tian, P Wei, F Yang, Z Lu, Z Chen, XA Liu, F Xu, L Wang Divergent neurocircuitry dissociates two components of the stress response: glucose mobilization and anxiety-like behavior Cell Reports, 2022-11-08;41(6):111586. 2022-11-08 [PMID: 36351404]
• Kurz E, Hirsch C, Dalton T et al. Exercise-induced engagement of the IL-15/IL-15R alpha axis promotes anti-tumor immunity in pancreatic cancer Cancer Cell 2022-06-01 [PMID: 35660135] (ELISA, Mouse)
• Alex A, Morten F, Andreas V et al. Oral delivery of systemic monoclonal antibodies, peptides and small molecules using gastric auto-injectors. Nat Biotechnol. 2021-08-30 [PMID: 34462588]
• Marina D, Carolyn C, Jasmin G et al. Leptin treatment prevents impaired hypoglycemic counterregulation induced by exposure to severe caloric restriction or exposure to recurrent hypoglycemia. Auton Neurosci. 2021-07-16 [PMID: 34358845]
• Arbnore G, Seryozha G, Vujica Z et al. The Effect of Aerobic Physical Activity in Adrenaline Level in White Laboratory Rats. Med Arch. 2020-04-07 [PMID: 32577046]
• L Lu, Y Ren, T Yu, Z Liu, S Wang, L Tan, J Zeng, Q Feng, R Lin, Y Liu, Q Guo, M Luo Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus Nat Commun, 2020-01-14;11(1):262. 2020-01-14 [PMID: 31937768]
• Berger J, Singh P, Khrimian L et al. Mediation of the Acute Stress Response by the Skeleton. Cell Metab. 2019-09-12 [PMID: 31523009]
• Lansdown A, Warnert E, Sverrisdottir Y et al. Regional Cerebral Activation Accompanies Sympathoexcitation in Women With Polycystic Ovary Syndrome. J Clin Endocrinol Metab. 2019-09-01 [PMID: 31127833]
• Seung-Jin K, Dechun F, Adrien G et al. Adipocyte Death Preferentially Induces Liver Injury and Inflammation Through the Activation of Chemokine (C-C Motif) Receptor 2-Positive Macrophages and Lipolysis Hepatology. 2019-03-18 [PMID: 30681731]
• Reno CM, Skinner A, Bayles J et al. Severe Hypoglycemia-Induced Sudden Death is Mediated by Both Cardiac Arrhythmias and Seizures. Am J Physiol Endocrinol Metab 2018-02-27 [PMID: 29486140]

FAQs for Epinephrine ELISA Kit (KA1882). (Showing 1 - 4 of 4 FAQs).

1. One of our customer would be interested in your kits: Dopamine ELISA Kit (KA1887) and Epinephrine ELISA Kit (KA1882). The customer is going to test mouse serum and plasma samples. Please, let me know if these kits could be use with mouse samples.
   • Both of these kits can be used with mouse urine and plasma samples very successfully.
2. My sample is serum from human being. I want to know whether the kit(KA1882) can detect my sample?
   • This ELISA kit is made by Abnova. We are their distributor in the United States. Their ELISA kit is an enzyme immunoassay for the quantitative determination of Adrenaline (Epinephrine) in plasma and urine. Other sample types have not yet been tested or validated. Since the kit is designed to detect epinephrine, there is no specificity with the kit. I will include a link to the protocol included with this ELISA kit for your reference: KA1882 Protocol.
3. I would like to know if the next kit is a general specie kit or is it human specific. Our interest is in a chicken Epinephrine ELISA kit but also a general one.
   • In principle KA1882 can be used with samples from chicken, and that cross-reactivity should not be an issue. The lab did, however, raise a concern as to whether the levels of chicken epinephrine would fall within the detection limits of the kit, and they suggested that the customer may wish to consult the literature regarding this.
4. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.