EndTag™ DNA/RNA End Labeling Kit

In-situ Hybridization: EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB] - Fluorescence in situ hybridization of human chromosomes using 5' EndTag Fluorescein-labeled puC1.77 detected directly and mounted in VECTASHIELD Mounting Media with PI.

EndTag(TM) DNA/RNA End Labeling Kit [MB-9001-NB]

• Applications: Func

EndTag™ DNA/RNA End Labeling Kit Summary

• Description: End labeling is a favored method for applications in which an internal label might interfere with hybridization or sequence specific protein binding. Features:
  
  
• Enables simple and uniform labeling of the 5' end of a DNA, RNA or unmodified oligonucleotide molecule.
• Preserves the unmodified 3' end of the oligonucleotide (ideal for the labeling of PCR primers as a result).
• Compatible with a choice of diverse labels - fluorophores, biotin, haptens, or affinity ligands*
• Kit Type: Labeling Kit

Applications/Dilutions

• Dilutions:
  • In-situ Hybridization
• Application Notes: Materials suitable to perform 10 end labeling reactions of up to 0.6 nmols of 5' ends (e.g. about 5 ug of a 25 base oligo) per reaction. *Note: Thiol-reactive labeling reagent is not included in the kit and must be purchased separately. Labels suitable for this kit include: Biotin (Long Arm) Maleimide, DNP Maleimide, Fluorescein (Long Arm) Maleimide, Fucose Maleimide, Texas Red(R) Maleimide..

Packaging, Storage & Formulations

• Storage: Store at -20 °C.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.