Endothelial Cells Antibody (OX-43)

Flow Cytometry: Endothelial Cells Antibody (OX-43) [NB100-65425] - Analysis using the FITC conjugate of NB100-65425. staining of rat peritoneal macrophages.

Product Discontinued

Endothelial Cells Antibody (OX-43) Summary

• Immunogen: Rat peritoneal macrophages
• Specificity: NB100-65425 recognizes an antigen of 90kD expressed on all vascular endothelial cells in the rat, with the exception of those in the brain. The antibody also recognizes resident and activated peritoneal macrophages, a subset of alveolar macrophages and erythrocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Endothelial Cells Antibody (OX-43)

• Endothelium

Background

The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endothelial Cells Antibody (NB100-65425)(2)

Publications using NB100-65425

• Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat)
• Lazo PA, Klein-Szanto AJ, Tsichlis PN. T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications. J Virol. [PMID: 2196385] (FLOW, Rat)