Endomorphin 1 and 2 Antibody

Immunohistochemistry: Endomorphin 1 and 2 Antibody [NB100-1656] - Endomorphin-1 and 2 staining in rat paraventricular thalamic nucleus.

Product Discontinued

Endomorphin 1 and 2 Antibody Summary

• Immunogen: Synthetic peptide conjugated to BSA.
• Specificity: Sensitivity has been determined by ELISA. Less than 1ng of could be detected after binding to Endomorphin 2 which is conjugated to titer plate:Endomorphin 2 = 100%. Endomorphin 1 = 70%. Leu5-Enkephalin = <0.01%. Met5-Enkephalin = <0.03%.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC-P. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Monkey and Pig.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole serum
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Endomorphin 1 and 2 Antibody

• C34H38N6O5
• EM-1

Background

Recently, endogenous agonists for the mu-opiate receptor, were first isolated from bovine brain [Nature,386, 499 (1997)]. These are endomorphin-1; Tyr-Pro-Trp-Phe-NH2, and endomorphin-2; Tyr-Pro-Phe-Phe-NH2. The isolation of relatively large amounts of endomorphin-1 and endomorphin-2 from human brain cortex has also reported [Peptides,18, 1635 (1997)]. The immunoreactivity for endomorphin was also detected in rat brain near the mu-opiate receptor containing neurons, but the molecular form of the rat peptide has not yet been elucidated [Peptides,18, 1641 (1997)]. These observations may be considered to indicate that endomorphin-1 and 2 are common mu-opiate agonists in mammals. In addition to the originally discovered mu-opiate agonistic function, these peptides have been reported to show hypotensive activity and Ca2+ channel current inhibitory activity [Biochem. Biophys. Res. Commun.,235, 567 (1997)]. Endomorphins would be useful for the study of pain perception together with some other biological functions in the body.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.