Recombinant eGFP His (N-Term) His (C-Term) Protein

• Applications: Func, PAGE
• Concentration: Lyoph

Recombinant eGFP His (N-Term) His (C-Term) Protein Summary

• Description: A recombinant protein with a N-Terminal and a C-terminal His-tag and corresponding to eGFP
  
  Source: E.coli Amino Acid Sequence: U57607.1 (NCBI Nucleotide)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab51992)
• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • In vitro assay
  • In vivo assay
  • SDS-Page
• Application Notes: Endotoxin level <0.1 ng/ug; suitable for in vivo injection or cell culture applications.
• Theoretical MW: 32.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Freeze Dried
• Preservative: No Preservative
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with PBS to 1 mg/ml

Alternate Names for Recombinant eGFP His (N-Term) His (C-Term) Protein

• Enhanced Green Fluorescent Protein
• GFP
• Green Fluorescent Protein

Background

The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody and GFP antibody on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for eGFP Recombinant Protein (NBP1-99915)(1)

Publication using NBP1-99915

• Sydor MJ, Serban MA., et Al. The Application of Fluorescence Anisotropy for Viscosity Measurements of Small Volume Biological Analytes J Exp Theor Anal 2024-04-12 [PMID: 38633433]