Human Echinococcus Antibody (IgG) ELISA Kit (Colorimetric) Summary
| Specificity |
This assay has high sensitivity and excellent specificity for detection of human echinococcus antibody IgG. No significant cross-reactivity or interference between human echinococcus antibody IgG and analogues was observed. |
| Standard Curve Range |
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| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 15% |
| Intra-Assay |
CV% < 15% |
| Sample Volume |
50-100ul |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Reactivity Notes
Please contact our Technical Services with any questions regarding species reactivity
Packaging, Storage & Formulations
Kit Components
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Components
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- Adhesive Strip (For 96 wells)
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Sample Diluent
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (10 x concentrate)
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Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells. Any antibodies specific for the antigen present will bind to the pre-coated antigen. Then add Horseradish Peroxidase (HRP) conjugated anti-human IgG antibody and incubated. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human echinococcus antibody IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Echinococcus Antibody (IgG) ELISA Kit (NBP2-59975). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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