EBOV Antibody (FE25)

Product Discontinued

EBOV Antibody (FE25) Summary

• Immunogen: Formalin inactivated Ebola virus (strain Zaire).
• Specificity: Ebola virus (FE25)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot
• Application Notes: In Western blot this Mab stains a single viral protein band corresponding to the envelope protein GP with a molecular mass of 74.5 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Background

Ebola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), VPX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.