E-Tag Epitope Tag Antibody [HRP]

Product Discontinued

E-Tag Epitope Tag Antibody [HRP] Summary

• Immunogen: Rabbits were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Western Blot 1:1000-1:10000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and 0.2% BSA
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for E-Tag Epitope Tag Antibody [HRP]

• E epitope tag
• GAPVPYPDPLEPR epitope tag
• GAPVPYPDPLEPR tag

Background

The E-tag is a popular epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized E-tag epitope represents the peptide sequence GAPVPYPDPLEPR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for E-Tag Epitope Tag Antibody (NB600-528). (Showing 1 - 1 of 1 FAQ).

1. Re-cat # NB600-527 , is this antibody the same as NB600-528 , only without the HRP ?
   • NB600-527 and NB600-528 were generated by immunising rabbits with E-Tag (GAPVPYPDPLEPR) conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on solid support. In the case of NB600-528 an HRP tag was then added.