E. Coli Antibody [HRP]

Product Discontinued

E. Coli Antibody [HRP] Summary

• Immunogen: Mixture of E. coli serotypes.
• Localization: Whole cell
• Specificity: Reacts with all "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is not absorbed for other members of the Enterobacteriaciae. Will remove E. coli proteins from recombinant preparations.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
• Application Notes: NOTE: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Cross-reacts with related Enterobacteriaceae. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 10 mg/ml BSA
• Preservative: 0.002% Thimerosal
• Concentration: 1.5 mg/ml
• Purity: Protein A purified

Alternate Names for E. Coli Antibody [HRP]

• Escherichia coli

Background

Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.