dsDNA IgM ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
12.5 to 200 U/mL
Sensitivity
1.0 U/mL

Order Details

dsDNA IgM ELISA Kit (Colorimetric) Summary

Standard Curve Range
12.5 to 200 U/mL
Sensitivity
1.0 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 5.2-12.4%
Intra-Assay
CV% < 3.1-6.4%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
dsDNA Ab IgM ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgM class autoantibodies against double-stranded DNA in human serum or plasma.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Enzyme conjugate solution (PBS, PROCLIN 300 <0.5% (v/v)), (light red) containingpolyclonal rabbit anti-human IgM; labelled with horseradish peroxidase. Ready touse.
  2. Divisible microplate consisting of 12 modules of 8 wells each, coated with humanrecombinant double-stranded DNA (dsDNA). Ready to use.
  3. Combined Calibrators with IgM class Anti-dsDNA antibodies (A-F) in a serum/buffermatrix (PBS, BSA, NaN3 <0,1% (w/w)) containing: IgM: 0; 12.5; 25; 50; 100; and 200U/ml. Ready to use.
  4. Anti-dsDNA Controls in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w))positive (1) and negative (2), for the respective concentrations see the enclosedpackage insert. Ready to use.
  5. Wash solution (PBS, NaN3 <0,1% (w/w)), concentrate (50x)
  6. TMB substrate solution. Ready to use.
  7. Sample buffer (Tris, NaN3 <0,1% (w/w)), yellow, concentrate (5x)
  8. Stop solution (contain acid). Ready to use.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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