dsDNA IgM ELISA Kit (Colorimetric) Summary
| Description |
dsDNA Ab IgM ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgM class autoantibodies against double-stranded DNA in human serum or plasma. |
| Standard Curve Range |
12.5 to 200 U/mL |
| Sensitivity |
1.0 U/mL |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 5.2-12.4% |
| Intra-Assay |
CV% < 3.1-6.4% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Anti-dsDNA Controls in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w))positive (1) and negative (2), for the respective concentrations see the enclosedpackage insert. Ready to use.
- Combined Calibrators with IgM class Anti-dsDNA antibodies (A-F) in a serum/buffermatrix (PBS, BSA, NaN3 <0,1% (w/w)) containing: IgM: 0; 12.5; 25; 50; 100; and 200U/ml. Ready to use.
- Divisible microplate consisting of 12 modules of 8 wells each, coated with humanrecombinant double-stranded DNA (dsDNA). Ready to use.
- Enzyme conjugate solution (PBS, PROCLIN 300 <0.5% (v/v)), (light red) containingpolyclonal rabbit anti-human IgM; labelled with horseradish peroxidase. Ready touse.
- Sample buffer (Tris, NaN3 <0,1% (w/w)), yellow, concentrate (5x)
- Stop solution (contain acid). Ready to use.
- TMB substrate solution. Ready to use.
- Wash solution (PBS, NaN3 <0,1% (w/w)), concentrate (50x)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for dsDNA IgM ELISA Kit (KA1099). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.