dsDNA IgM ELISA Kit (Colorimetric)


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Product Details

Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
12.5 to 200 U/mL
1.0 U/mL

Order Details

dsDNA IgM ELISA Kit (Colorimetric) Summary

Standard Curve Range
12.5 to 200 U/mL
1.0 U/mL
Assay Type
Sandwich ELISA
CV% < 5.2-12.4%
CV% < 3.1-6.4%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
dsDNA Ab IgM ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgM class autoantibodies against double-stranded DNA in human serum or plasma.

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Combined Calibrators with IgM class Anti-dsDNA antibodies (A-F) in a serum/buffermatrix (PBS, BSA, NaN3 <0,1% (w/w)) containing: IgM: 0; 12.5; 25; 50; 100; and 200U/ml. Ready to use.
  2. Divisible microplate consisting of 12 modules of 8 wells each, coated with humanrecombinant double-stranded DNA (dsDNA). Ready to use.
  3. Enzyme conjugate solution (PBS, PROCLIN 300 <0.5% (v/v)), (light red) containingpolyclonal rabbit anti-human IgM; labelled with horseradish peroxidase. Ready touse.
  4. Anti-dsDNA Controls in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w))positive (1) and negative (2), for the respective concentrations see the enclosedpackage insert. Ready to use.
  5. Stop solution (contain acid). Ready to use.
  6. TMB substrate solution. Ready to use.
  7. Wash solution (PBS, NaN3 <0,1% (w/w)), concentrate (50x)
  8. Sample buffer (Tris, NaN3 <0,1% (w/w)), yellow, concentrate (5x)


This product is produced by and distributed for Abnova, a company based in Taiwan.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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