dsDNA IgG ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
12.5 to 200 IU/mL |
| Sensitivity |
1.0 IU/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 5.2-12.4% |
| Intra-Assay |
CV% < 3.1-6.4% |
| Sample Volume |
10 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
ds-DNA Ab IgG ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against double-stranded DNA in human serum or plasma. |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Calibrator A-F (0; 12.5; 25; 50; 100; and 200 IU/ml), containing dsDNA antibodies ina serum/buffer matrix (PBS, BSA, detergent, NaN3 <0.09%), yellow. Ready to use.
- Control positive (1) and negative (2), containing dsDNA antibodies in a serum/buffermatrix (PBS, BSA, detergent, NaN3 <0.09%), yellow. Ready to use. Theconcentration is specified on the certificate of analysis.
- Divisible microplate: consisting of 12 modules of 8 wells each. Ready to use.
- Enzyme conjugate containing anti-human IgG antibodies, HRP labeled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.
- Sample Buffer PD, containing PBS, BSA, detergent, preservative NaN3 0.09%,yellow, concentrate (5x)
- Stop solution; contains acid. Ready to use.
- TMB substrate; containing 3,3’, 5, 5’-Tetramethylbenzidin, colorless. Ready to use.
- Wash solution, containing Tris, detergent, preservative NaN3 0.09%; 50x conc
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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