dsDNA IgG ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
12.5 to 200 IU/mL
Sensitivity
1.0 IU/mL

Order Details

dsDNA IgG ELISA Kit (Colorimetric) Summary

Standard Curve Range
12.5 to 200 IU/mL
Sensitivity
1.0 IU/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 5.2-12.4%
Intra-Assay
CV% < 3.1-6.4%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
ds-DNA Ab IgG ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against double-stranded DNA in human serum or plasma.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Sample Buffer PD, containing PBS, BSA, detergent, preservative NaN3 0.09%,yellow, concentrate (5x)
  2. TMB substrate; containing 3,3’, 5, 5’-Tetramethylbenzidin, colorless. Ready to use.
  3. Stop solution; contains acid. Ready to use.
  4. Wash solution, containing Tris, detergent, preservative NaN3 0.09%; 50x conc
  5. Control positive (1) and negative (2), containing dsDNA antibodies in a serum/buffermatrix (PBS, BSA, detergent, NaN3 <0.09%), yellow. Ready to use. Theconcentration is specified on the certificate of analysis.
  6. Calibrator A-F (0; 12.5; 25; 50; 100; and 200 IU/ml), containing dsDNA antibodies ina serum/buffer matrix (PBS, BSA, detergent, NaN3 <0.09%), yellow. Ready to use.
  7. Divisible microplate: consisting of 12 modules of 8 wells each. Ready to use.
  8. Enzyme conjugate containing anti-human IgG antibodies, HRP labeled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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