DRAQ9™

Fluorescence Imaging: DRAQ9 (TM) [NBP2-81128] - Cells stained with .

Live Imaging Microscopy: DRAQ9 (TM) [NBP2-81128] - DRAQ9 was diluted in EMEM culture media and applied to live HeLa cells at 2 uM (1:500) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.

DRAQ9 (TM) [NBP2-81128] - Excitation and Emission Spectra for DRAQ9 (TM).

Live Imaging Microscopy: DRAQ9 (TM) [NBP2-81128] - labelling of live U2OS cells for 48 h at 2 uM - showing a number of cells in mitosis.

Fluorescence Imaging: DRAQ9 (TM) [NBP2-81128] - 6-day U2OS spheroid with continuous labeling with at 2 uM.

• Applications: ICC/IF, MS, MS

DRAQ9™ Summary

• Description: Key Features of DRAQ9 (TM) :
  
• Far-red dye that stains membraneous structures in the cytoplasm but not the nucleus
• Live-cell, permeant dye that can be used for live or fixed cells
• Track cells through mitotic cell division and monitor spheroid/organoid cultures in real-time
• Non-toxic and optimal for long-term imaging studies, up to several days
• Used for cell painting or mosaic analysis in high content phenotypic screening
• Spectrally compatible with GFP/FITC and UV-excited probes such as Hoechst


Products are shipped at ambient temperature, but on receipt packs should be stored at -20C. Thaw and adjust temperature to above 20C before use.
• Purity: >97%

Applications/Dilutions

• Dilutions:
  • Fluorescence Imaging 1:50 - 1:500
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Live Imaging Microscopy 1:500
• Application Notes: DRAQ9 (TM) is supplied as a DMSO solution at a concentration of 1mM: the 200 ul size equates to 8x 24-multi-well plates and 5x 96-microtiter plates. The 1 ml size equates to 40x 24-multi-well plates, 25x 96-microtiter plates, and 3.3x 384-microtiter plates.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Purity: >97%

Notes

DRAQ9 (TM) is a registered trademark of BioStatus Limited.

Background

DRAQ9 (TM) is a far-red fluorescent dye that labels membranous and vesicular structures in the cytoplasm but not the cell nucleus. DRAQ9 (TM) does not crosslink with or esterify cytoplasmic proteins. Its excitation wavelength maxima are 605 nm / 655 nm and its emission wavelength maximum is 697 nm when bound to membranous structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for DRAQ9 (TM) (NBP2-81128)(1)

Publication using NBP2-81128

• Yosief R, Nebbioso G, Koshkin V et al. Making Cytometry of Reaction Rate Constant (CRRC) Applicable to Motile Cells ChemRxiv 2022-04-21 (CS)