Dopamine ELISA Kit (Colorimetric)

• Reactivity: Hu, Rt, Fi, I
• Applications: ELISA, NULL
• Conjugate: HRP
• Suitable Sample Type: Biological Fluid
• Standard Curve Range: 0.5 ng/ml to 80 ng/ml
• Sensitivity: 0.25 pg/mL x Correction factor

Dopamine ELISA Kit (Colorimetric) Summary

• Description: Dopamine ELISA Kit is an ultra-sensitive enzyme immunoassay for the quantitative determination of Dopamine in biological fluids.
• Specificity: Dopamine ELISA Kit is an ultra-sensitive enzyme immunoassay for the quantitative determination of Dopamine in biological fluids.
• Standard Curve Range: 0.5 ng/ml to 80 ng/ml
• Sensitivity: 0.25 pg/mL x Correction factor
• Assay Type: Colorimetric
• Intra-Assay: Human EDTA-Plasma: 15.6%, Cell Culture Medium: 18.4%
• Spike Recovery: Human EDTA-Plasma: 97.7%, Cell Culture Medium: 98.6%
• Sample Volume: 1-750 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dopamine ELISA Kit (Colorimetric)

• 3 4 dihydroxyphenethylamine
• 3 hydroxytyramine
• DA
• Dopamine
• Oxytyramine

Background

Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Dopamine Kit (KA3838)(6)

Publications using KA3838

• Smith L, Ryde I, Hartman J et al. Strengths and limitations of morphological and behavioral analyses in detecting dopaminergic deficiency in Caenorhabditis elegans. Neurotoxicology. 2019-07-16 [PMID: 31323240]
• Gutbier S, May P, Berthelot S et al. Major changes of cell function and toxicant sensitivity in cultured cells undergoing mild, quasi-natural genetic drift. Arch Toxicol 2017-10-08 [PMID: 30298209]
• Massarsky A, Jayasundara N, Glazer L et al. Outcomes of developmental exposure to total particulate matter from cigarette smoke in zebrafish (Danio rerio). Neurotoxicology 2018-07-17 [PMID: 30026038]
• Simanjuntak Y, Liang JJ, Lee YL et al. Japanese Encephalitis Virus Exploits Dopamine D2 Receptor-phospholipase C to Target Dopaminergic Human Neuronal Cells. Front Microbiol 2017-04-11 [PMID: 28443089]
• Park M, Shin S, Cheng J et al. Nanocellulose based asymmetric composite membrane for the multiple functions in cell encapsulation. Carbohydr Polym 2016-12-28 [PMID: 28024536]
• Gao L, Zhou W, Symmes B, Freed CR Re-Cloning the N27 Dopamine Cell Line to Improve a Cell Culture Model of Parkinson's Disease. PLoS One 2016-08-11 [PMID: 27512998] (WB)