Dopamine ELISA Kit (Colorimetric)

ELISA: Dopamine ELISA Kit [KA1887] - Standard Curve Note: The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

• Applications: ELISA, NULL
• Suitable Sample Type: Plasma, Urine
• Standard Curve Range: Urine: 4.8 to 2000 ng/mL, Plasma: 75 to 33,333 pg/mL
• Sensitivity: Urine: 2.5 ng/mL, Plasma: 49 pg/mL

Dopamine ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. Dopamine ELISA Kit is an enzyme immunoassay for the quantitative determination of Dopamine in plasma and urine.
• Standard Curve Range: Urine: 4.8 to 2000 ng/mL, Plasma: 75 to 33,333 pg/mL
• Sensitivity: Urine: 2.5 ng/mL, Plasma: 49 pg/mL
• Assay Type: Quantification
• Inter-Assay: CV% < 17.05%
• Intra-Assay: CV% < 12.65%
• Spike Recovery: Urine: 95%, Plasma: 90%
• Sample Volume: Extracted urine samples: 25 uL, Extracted plasma samples: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dopamine ELISA Kit (Colorimetric)

• 3 4 dihydroxyphenethylamine
• 3 hydroxytyramine
• DA
• Dopamine
• Oxytyramine

Background

Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Dopamine ELISA Kit (KA1887)(31)

Publications using KA1887

• Stefano G, Germana C, Alessandro M et al. Natural killer cells and innate lymphoid cells 1 tune anxiety-like behavior and memory in mice via interferon-? and acetylcholine. Nat Commun. 2023-05-29 [PMID: 37248289]
• Jun L, Nan L, Jingkuan W et al. Genetically engineered mesenchymal stem cells with dopamine synthesis for Parkinson's disease in animal models. NPJ Parkinsons Dis. 2022-12-22 [PMID: 36550118]
• Abayomi A, Benneth B, Gracious O et al. Repeated social defeat stress exacerbates lipopolysaccharide-induced behavioural deficits in mice: ameliorative role of Chrysophyllum albidum fruit extract through anti-neuroinflammation, antioxidant and neurochemical balance. Metab Brain Dis. 2022-07-22 [PMID: 35867181]
• Rana D, Colombani T, Mohammed H et al. Strategies to prevent dopamine oxidation and related cytotoxicity using various antioxidants and nitrogenation emergent mater. 2019-06-01
• Soheila Siroosbakht, Sadra Rezakhaniha, Bijan Rezakhaniha Are androstenedione, dihydrotestosterone, thyroid-stimulating hormone, insulin-like growth factor I, and insulin-like growth factor binding protein 3 necessary for isolated micropenis healthy boys' evaluation without any phenotypic abnormalities? A cross-sectional study. Andrologia 2022-12-02 [PMID: 36257721]
• Ji K, Oc-Hee K, In R et al. Challenge exposure to whole cigarette smoke condensate upregulates locomotor sensitization by stimulating ?4?2 nicotinic acetylcholine receptors in the nucleus accumbens of rats. Pharmacol Biochem Behav. 2022-09-30 [PMID: 36183870]
• Roland A, Oladele A, Ayodeji A et al. L-Arginine reverses maternal and pre-pubertal codeine exposure-induced sexual dysfunction via upregulation of androgen receptor gene and NO/cGMP signaling. PLoS One. 2022-09-13 [PMID: 36099318]
• Viktor S, Oleg V, Ekaterina T et al. Prenatal Hypoxia Affects Nicotine Consumption and Withdrawal in Adult Rats via Impairment of the Glutamate System in the Brain. Mol Neurobiol. 2022-05-17 [PMID: 35581520]
• Sunghyun K, Sumin S, In R et al. Nicotine rather than non-nicotine substances in 3R4F WCSC increases behavioral sensitization and drug-taking behavior in rats. Nicotine Tob Res. 2022-03-22 [PMID: 35323980]
• Alamri HS Talbinah (Barley Porridge): neurotransmitters modulatory effect and antidepressant-like action in experimentally depressed rats European review for medical and pharmacological sciences 2022-01-01 [PMID: 35048996] (ELISA)
• Arvie A, Raly C, Chrislean B et al. 1-phenylcyclohexan-1-amine hydrochloride (PCA HCl) alters mesolimbic dopamine system accompanied by neuroplastic changes: A neuropsychopharmacological evaluation in rodents. Neurochem Int. 2021-01-15 [PMID: 33460722]
• Kasthuri M, Ammu R, Sushela S et al. Influence of serum concentration in retinoic acid and phorbol ester induced differentiation of SH-SY5Y human neuroblastoma cell line. Mol Biol Rep. 2020-10-23 [PMID: 33098048]
• Fang L, Sushan L, Wenjing Q et al. Developmental deficits and early signs of neurodegeneration revealed by PD patient derived dopamine neurons. Stem Cell Res. 2020-10-05 [PMID: 33059129]
• Raly C, Leandro S, Chrislean B et al. Two newly-emerging substituted phenethylamines MAL and BOD induce differential psychopharmacological effects in rodents. J Psychopharmacol. 2020-07-10 [PMID: 32648801]
• ark S, Lee J, Seo S et al. High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis. Sci Rep. 2020-02-28 [PMID: 32111933]
• Amos A, Kehinde F, Chizim I et al. Curcumin attenuates copper-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Toxicol Rep. 2020-01-27 [PMID: 32025502]
• Abiero A, Botanas C, Custodio R et al. 4-MeO-PCP and 3-MeO-PCMo, new dissociative drugs, produce rewarding and reinforcing effects through activation of mesolimbic dopamine pathway and alteration of accumbal CREB, deltaFosB, and BDNF levels. Psychopharmacology (Berl). 2019-12-11 [PMID: 31828394]
• Khedr N, Ebeid A, Khalil R et al. New insights into weight management by orlistat in comparison with cinnamon as a natural lipase inhibitor. Endocrine. 2019-11-13 [PMID: 31721089]
• Sun M, Shen X, Ma Y et al. Rehmannioside A attenuates cognitive deficits in rats with vascular dementia (VD) through suppressing oxidative stress, inflammation and apoptosis. Biomed Pharmacother. 2019-10-05 [PMID: 31593895]
• Alabi A, Ajayi A, Ben-Azu B et al. Methyl jasmonate ameliorates rotenone-induced motor deficits in rats through its neuroprotective activity and increased expression of tyrosine hydroxylase immunopositive cells. Metab Brain Dis. 2019-08-28 [PMID: 31463866]
• Hebron M, Javidnia M, Moussa C et al. Tau clearance improves astrocytic function and brain glutamate-glutamine cycle. J Neurol Sci. 2018-06-12 [PMID: 30103978]
• Lee HT, Lee KI, Lin HC et al. Genetic Deletion of Soluble Epoxide Hydroxylase Causes Anxiety-Like Behaviors in Mice. Mol Neurobiol 2018-07-23 [PMID: 30033504]
• Liu Z, Wang L, Yan Y et al. D1 dopamine receptor is involved in shell formation in larvae of Pacific oyster Crassostrea gigas. Dev Comp Immunol 2018-03-14 [PMID: 29550270]
• Chiu Chun-Hung, Chyau Charng-Cherng, Chen Chin-Chu et al. Erinacine A-Enriched Hericium erinaceus Mycelium Produces Antidepressant-Like Effects through Modulating BDNF/PI3K/Akt/GSK-3 beta Signaling in Mice. Cell 2019-01-24 [PMID: 29364171] (ELISA, Mouse)
• Nakamura TY, Nakao S, Nakajo Y et al. Possible Signaling Pathways Mediating Neuronal Calcium Sensor-1-Dependent Spatial Learning and Memory in Mice. PLoS One 2017-01-25 [PMID: 28122057]
• Lenis YY, Wang X, Tang W et al. Effects of agmatine on secretion of interferon tau and catecholamines and expression of genes related to production of polyamines by ovine trophectoderm cells. Amino Acids 2016-04-13 [PMID: 27074718]
• Huang CL, Chao CC, Lee YC et al. Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability. Mol Neurobiol. 2015-05-07 [PMID: 25947082]
• Yang F, Liu Y, Tu J et al. Activated astrocytes enhance the dopaminergic differentiation of stem cells and promote brain repair through bFGF. Nat Commun. 2014-12-17 [PMID: 25517983]
• Hiramoto K, Yamate Y, Kawanishi S. Detection of Dopa-positive cells in mouse ovaries in response to ocular exposure to ultraviolet B rays. Photodermatol Photoimmunol Photomed. 2014-10-24 [PMID: 25345490]
• Lonskaya I, Desforges NM, Hebron ML, Moussa CE. Ubiquitination Increases Parkin Activity to Promote Autophagic alpha-Synuclein Clearance. PLoS One. 2013-12-26 [PMID: 24386307]
• Hebron ML, Lonskaya I, Moussa CE. Nilotinib reverses loss of dopamine neurons and improves motor behavior via autophagic degradation of alpha-synuclein in Parkinsons disease models. Hum Mol Genet. 2013-05-10 [PMID: 23666528]

FAQs for Dopamine ELISA Kit (KA1887). (Showing 1 - 4 of 4 FAQs).

1. One of our customer would be interested in your kits: Dopamine ELISA Kit (KA1887) and Epinephrine ELISA Kit (KA1882). The customer is going to test mouse serum and plasma samples. Please, let me know if these kits could be use with mouse samples.
   • Both of these kits can be used with mouse urine and plasma samples very successfully.
2. I wanted some information regarding the ELISA kit form Novus biologicals cat no. KA1887 and NB100-64534. Its sensitivity and detection towards lowest levels of dopamine and cross reactivity. Can this product be used for quantifying DOPAMINE from culture supernatant?
   • KA1887 has been tested on urine and plasma and the limit of detection is 4.5 ng/mL urine and 20 pg/mL plasma. It will most likely work on cell culture supernatant as well. NB100-64534 is just an antibody that you would need to use to create your own ELISA, so the limit of detection has not been determined.
3. Will this ELISA work for tissue homogenates?
   • Thank you for contacting Novus Biologicals. KA1887 is made by Abnova, for whom we distribute in the US. This kit has been QC'd using plasma and urine. Abnova has not tested this for other samples and does not guarantee it for use on those samples. I have included a link to the protocol for your perusal. KA1887 Protocol.
4. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.