DNA/RNA Damage Antibody (15A3) - BSA Free

Immunocytochemistry/Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878] - visualized on a retinal injury

Immunohistochemistry: DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial.

DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

• Reactivity: Hu, Mu, All-NA
• Applications: DB, ELISA, Flow, ICC/IF, IHC, IP, Func
• Clone: 15A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DNA/RNA Damage Antibody (15A3) - BSA Free Summary

• Immunogen: 8-hydroxy-guanosine-BSA and-casein conjugates
• Specificity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Functional Assay
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
• Application Notes: Use in immoprecipitation reported in scientific literature (PMID 26510519). Use in Immunoassay reported in scientific literature (PMID:31625228).

Reactivity Notes

Use in Human reported in scientific literature (PMID:33482333) Mouse reactivity reported in scientific literature (PMID: 31226694).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for DNA/RNA Damage Antibody (15A3) - BSA Free

• DNA/RNA Oxidative Damage Markers Monoclonal Antibody

Background

DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA/RNA Damage Antibody (NB110-96878)(19)

Publications using NB110-96878

• Jie Tang, Xiaoxue Lu, Tao Zhang, Yuyang Feng, Qiaolin Xu, Jing Li, Yuanzhi Lan, Huaxing Luo, Linghai Zeng, Yuanyuan Xiang, Yan Zhang, Qian Li, Xuhu Mao, Bin Tang, Dongzhu Zeng Shiga toxin 2 A-subunit induces mitochondrial damage, mitophagy and apoptosis via the interaction of Tom20 in Caco-2 cells Heliyon 2023-09-09 [PMID: 37809632]
• Fraser BA, Wilkins AL, De Iuliis GN et al. Development of a model for studying the developmental consequences of oxidative sperm DNA damage by targeting redox-cycling naphthoquinones to the Sertoli cell population Free radical biology & medicine 2023-06-23 [PMID: 37356777] (Immunohistochemistry, Mouse)
• Langford MP, Srur L, Redens TB, Byrd WA Conjunctival epitheliopathy induced by topical exposure to bacterial peptidoglycan, muramyl dipeptide Experimental eye research 2023-01-10 [PMID: 36634837] (IHC-P, ICC/IF, Rabbit)
• Soria-Meneses PJ, Jurado-Campos A, G�mez-Rubio V et al. Determination of Ram (Ovis aries) Sperm DNA Damage Due to Oxidative Stress: 8-OHdG Immunodetection Assay vs. SCSA(�) Animals (Basel) 2022-11-25 [PMID: 36496807] (Immunohistochemistry, Mouse)
• Shrestha S, Erikson G, Lyon J et al. Aging compromises human islet beta cell function and identity by decreasing transcription factor activity and inducing ER stress Science advances 2022-10-07 [PMID: 36197983] (IHC-P, Human)
• Gonzalez M, Prashar T, Connaughton H et al Restoring Sperm Quality Post-Cryopreservation Using Mitochondrial-Targeted Compounds Antioxidants (Basel) 2022-09-14 [PMID: 36139882] (Immunocytochemistry/ Immunofluorescence, Human)
• Sadeghi N, Tavalaee M, Kiani-Esfahani A Et al. Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation Basic and clinical andrology 2021-10-21 [PMID: 34670490] (FLOW)
• Berby B, Bichara C, Rives-Feraille A et al. Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males Antioxidants (Basel, Switzerland) 2021-04-12 [PMID: 33921485]
• Zhao F, Whiting S, Lambourne S, et al. Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa Free radical biology & medicine 2021-01-19 [PMID: 33482333] (ICC/IF, Human)
• Aitken R, Whiting S, Connaughton H et al. A novel pathway for the induction of DNA damage in human spermatozoa involving extracellular cell-free DNA Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2020-09-01 [PMID: 32920458] (Human)
• Champroux A, Goubely C, Henry-Berger J et al. Three-Dimensional Confocal Analysis of Chromosome Positioning Coupled with Immunofluorescence in Mouse Sperm Nuclei Methods Mol. Biol. 2020-08-22 [PMID: 32822037]
• Alissafi T, Kalafati L, Lazari M et al. Mitochondrial Oxidative Damage Underlies Regulatory T Cell Defects in Autoimmunity Cell Metab. 2020-07-22 [PMID: 32738205]
• Noblanc, A, Klaassen, A., Robaire, B The Exacerbation of Aging and Oxidative Stress in the Epididymis of Sod1 Null Mice Antioxidants (Basel) 2020-02-11 [PMID: 32054065] (IHC-P, Mouse)
• Soria-Meneses PJ, Jurado-Campos A, Montoro V et al. Ovine sperm DNA oxidation quantification using an 8-OHdG immunodetection assay Reprod. Domest. Anim. 2019-10-01 [PMID: 31625228] (IA)
• Youngson NA, Uddin GM, Das A et al. Impacts of obesity, maternal obesity and nicotinamide mononucleotide supplementation on sperm quality in mice Reproduction 2019-08-01 [PMID: 31226694] (ICC/IF, Mouse)
• Vorilhon S, Brugnon F et al. Accuracy of human sperm DNA oxidation quantification and threshold determination using an 8-OHdG immuno-detection assay. Hum Reprod 2018-01-04 [PMID: 29579272] (FLOW, Human)
• Xavier MJ, Nixon B, Roman SD, Aitken RJ. Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage. PLoS ONE 2018-03-26 [PMID: 29579126] (IP)
• Oblette A, Rives N, Dumont L et al. Assessment of sperm nuclear quality after in vitro maturation of fresh or frozen/thawed mouse pre-pubertal testes Mol Hum Reprod 2017-10-01 [PMID: 28962037]
• Noblanc A, Peltier M, Damon-SoubeyrC et al. Epididymis Response Partly Compensates for Spermatozoa Oxidative Defects in snGPx4 and GPx5 Double Mutant Mice PLoS One 2012-01-01 [PMID: 22719900] (IF/IHC, Mouse)

FAQs for DNA/RNA Damage Antibody (NB110-96878). (Showing 1 - 2 of 2 FAQs).

1. Would you please help check if the description of Specificity on the product page of NB110-96878 is complete? We would like to ensure the antibody can recognize both 8OHdG and 8OHG
   • Yes, it is reactive to both 8-hydroxy-2’-deoxyguanosine (8OHdG) and 8-hydroxyguanine or 8-hydroxyguanosine (8OHG).
2. What is the concentration of this antiboby in mol/ml units or give us the molar mass?
   • Unfortunately, we do not calculate the molar concentration or mass of our antibodies. This antibody is provided as 0.1mg at 1.0mg/ml.