DNA Damage Quantification Assay Kit (Colorimetric)

Product Discontinued

DNA Damage Quantification Assay Kit (Colorimetric) Summary

• Description: Rapid and convenient kit to quantify Apurinic/Apyrimidinic (AP) sites. The kit provides the necessary reagents for determination of abasic sites in purified DNA samples in 96-well plate format.
  
  Detection method: Absorbance (450 and 650 nm) Applications: Determination of DNA damage (abasic sites) in sample DNA.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab284577-25)
• Kit Type: Assay Kit (Colorimetric)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background

Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed during the course of base excision and repair of oxidized, deaminated or alkylated bases. It has been estimated that about 2x10^5 base lesions are generated per cell per day. The level of AP sites in cells can be a good indicator of DNA lesion and repair against chemical damage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the openring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assayfollowed by a colorimetric detection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.