Diphtheria Toxin Antibody (8G1)

Product Discontinued

Diphtheria Toxin Antibody (8G1) Summary

• Immunogen: Native protein
• Specificity: This product recognises diphtheria toxin (DT), secreted by certain strains of Corynebacterium diphtheriae. DT catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kDa, it is then processed to form A and B fragments both of which appear to be required for toxicity. This product is also specific for the intact toxin/toxoid and does not react with free A or B subunits.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000

Reactivity Notes

Bacteria. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Diphtheria Toxin Antibody (8G1)

• Diphtheria Toxin
• tox

Background

Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.