Diphtheria Toxin A Antibody (8A4)

Product Discontinued

Diphtheria Toxin A Antibody (8A4) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid.
• Specificity: This reacts with free A subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin or free B subunit of Diphtheria toxin.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 1:100-1:2000
• Application Notes: Detection of Diphtheria toxin A-subunit by different immunochemical techniques. Recommend pair for sandwich immunoassay (capture-detection): NB110-2565 - NB110-2567.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Diphtheria Toxin A Antibody (8A4)

• Diphtheria Toxin

Background

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Diphtheria Toxin A Antibody (NB110-2565). (Showing 1 - 1 of 1 FAQ).

1. Would you be able to confirm the specificity (for only free A subunits of DTX) and isotype (Mouse IgG2a) of NB110-2565 - Mouse Monoclonal anti-Diphtheria toxin A-subunit (8A4)?
   • Our Diphtheria Toxin A antibody with catalogue number NB110-2565 was raised by generating hybridoma clones following hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid. The antibody reacts with free A-subunits of Diphtheria toxin, but does not react with the whole Diphtheria toxin and free B-subunit of Diphtheria toxin.