Dibromo-tyrosine ELISA Kit (Colorimetric)

ELISA: Dibromo-tyrosine ELISA Kit (Colorimetric) [NBP2-61928] - Typical Standard Curve for the Dibromo-tyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.078 -5 ug/mL.

ELISA: Dibromo-tyrosine ELISA Kit (Colorimetric) [NBP2-61928] - Recovery of dibromo-tyrosine from urine. Urine samples were spiked with dibromo-tyrosine, and diluted as described in the Sample Preparation section of the manual (page 10) and analyzed using the Dibromo-Tyrosine ELISA kit. The y-intercept corresponds to the amount of dibromo-tyrosine in unspiked urine.

ELISA: Dibromo-tyrosine ELISA Kit (Colorimetric) [NBP2-61928] - Graph of the Dibromo-tyrosine Spike Recovery in Human Plasma.

ELISA: Dibromo-tyrosine ELISA Kit (Colorimetric) [NBP2-61928] - Graph of the sample comparison between diabetic smoker plasma and non-diabetic/non-smoker plasma.

Product Discontinued

Dibromo-tyrosine ELISA Kit (Colorimetric) Summary

• Specificity: ELISA kit used to quantify free and protein-bound 3,5-dibromotyrosine in samples. No detectable cross-reactivity was observed at assay range 0.078 ug/mL to 5 ug/mL for tyrosine, di-tyrosine, 3-nitro-tyrosine or 3-bromo-tyrosine. Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the Dibromo-tyrosine ELISA has been determined to be < 10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the Dibromo-tyrosine ELISA has been determined to be < 10%.
• Standard Curve Range: 0.078 - 5 ug/mL
• Sensitivity: 0.04 ug/mL
• Assay Type: Competitive ELISA (Enzyme-linked Immunosorbent Assay)
• Spike Recovery: Spike recovery and linearity of dilution studies yielded 10% precision across dilutions
• Sample Volume: 39 samples in duplicate, 23 in triplicate
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Dibromo-tyrosine ELISA Kit (Colorimetric)

• 5-dibromotyrosine;5-Dibromo-tyrosine;Dibromotyrosine;Dibromo-tyrosine;DiBrY

Background

Dibromo-tyrosine is produced by the oxidative bromination of tyrosine residues. This reaction occurs via eosinophil peroxidase (EPO), an enzyme released by activated eosinophils. Upon activation of eosinophils, a respiratory burst occurs releasing elevated levels of O2 and H202. In the oxidation of tyrosine, EPO utilizes H202 to catalyze the peroxidation of physiological levels of bromine found within plasma to generate the brominating reagent hypobromous acid (HOBr)(1-5). Eosinophils play an immunomodulatory role through their recruitment to host sites of parasitic invasion. EPO levels also contribute to diseases such as asthma, cancers and allergic disorders where cellular activation is found to occur at pathological sites (6-10). Brominated products such as 3,5-dibromo-tyrosine serve as biological markers for in vivo eosinophil-mediated tissue damage which allows for understanding the overall roll oxidative stress has on pathways implicated in diseased states within organisms (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Dibromo-tyrosine ELISA Kit (NBP2-61928). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.