DHT ELISA Kit (Colorimetric)

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ELISA: DHT ELISA Kit [KA1886] - Typical calibrator curve

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1886
    • Availability
      Product Discontinued

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DHT ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard curve. Dihydrotestosterone ELISA Kit is an enzyme immunoassay for the quantitative determination of Dihydrotestosterone in human serum.
Standard Curve Range
0 to 300 pg/mL
Sensitivity
6 pg/mL
Assay Type
Quantification
Inter-Assay
CV% < 8.5%
Intra-Assay
CV% < 6.92%
Spike Recovery
0.9057
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.
Publications
Read Publications using KA1886.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Biotinylated Rat BNP-45 Antibody (50x): A 50-fold biotinylated polyclonal antibodyagainst rat BNP-45.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. MIX Diluent Concentrate (10x): A 10-fold buffered protein base.
  4. Rat BNP-45 Microplate: A 96 well polystyrene microplate coated with a polyclonalantibody against rat BNP-45.
  5. Rat BNP45 Standard: Rat BNP-45 in a buffered protein base (lyophilized).
  6. Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
  7. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DHT ELISA Kit (KA1886). (Showing 1 - 2 of 2 FAQs).

  1. Have you ever tested this kit on a DSX ELISA Analyzer?
    • This kit has not yet been tested with a DSX ELISA analyzer.
  2. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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