Dengue Virus NS1 protein Antibody (973604) [Alexa Fluor® 700]

• Reactivity: V
• Applications: ELISA
• Clone: 973604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Dengue Virus NS1 protein Antibody (973604) [Alexa Fluor® 700] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived viral Dengue Virus 1 NS1 protein
  Asp776-Ala1127
  Accession # P17763
• Specificity: Detects viral Dengue Virus 1 NS1 protein in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dengue Virus NS1 protein Antibody (973604) [Alexa Fluor® 700]

• DEN-1
• Dengue Virus 1 NS1
• Dengue virus Serotype 1
• Dengue virus Type 1
• DENV1
• DENV1-NS1

Background

Dengue virus type 1 non-structural protein 1 (DENV1 NS1)  is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV1 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3).Mature DENV1 NS1 shares 73%, 80%, and 69% aa sequence identity with DENV2, DENV3, and DENV4 NS1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P17763()