Dendritic Cell Marker Antibody (33D1) [PE]

Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [PE] [NBP2-00153] - Staining of BALB/c splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.125 ug of Rat IgG2b kappa Isotype Control PE (left) or 0.125 ug of Anti-Mouse Dendritic Cell Marker (33D1) PE (right). Total viable cells were used for analysis.

Product Discontinued

Dendritic Cell Marker Antibody (33D1) [PE] Summary

• Immunogen: The immunogen for this antibody was Dendritic Cell Marker.
• Marker: Dendritic Cell Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Dendritic Cell Marker Antibody (33D1) [PE]

• DCs
• Dendritic cell

Background

The 33D1 monoclonal antibody reacts with a mouse DC-specific surface marker. The nature and biological activity of the 33D1 antigen is as of yet unknown. 33D1 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express the 33D1 antigen and this expression is down regulated in the presence of IL-4. 33D1 antigen has been detected in vivo in brain dendritic cells post infection via Toxoplasma gondii.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.