DDX59 Overexpression Lysate (Native)


Western Blot: DDX59 Overexpression Lysate (Adult Normal) [NBL1-09809] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX59.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

DDX59 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC207173 and based on accession number NM_001031725. The protein contains a C-terminal DDK tag.
Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 59 (DDX59), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
68.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for DDX59

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DDX59 Overexpression Lysate (Native)

  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
  • DEAD box protein 59
  • DKFZP564B1023
  • EC 3.6.1
  • EC
  • Zinc finger HIT domain-containing protein 5
  • ZNHIT5probable ATP-dependent RNA helicase DDX59


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional DDX59 Overexpression Lysate (Native) Products

Bioinformatics Tool for DDX59 Lysate (NBL1-09809)

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Contact Information

Product PDFs


Gene Symbol DDX59

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