![Western Blot: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59.](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Western-Blot-NBP2-72229-img0001.jpg "Western Blot: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI2G1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | Novus Biologicals Mouse DDX59 Antibody (OTI2G1) - Azide and BSA Free (NBP2-46447) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-303 of human DDX59 (NP_001026895) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DDX59 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 68.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DDX59 |
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0015.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0002.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0003.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0004.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0005.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0006.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0007.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0008.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0009.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0010.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0011.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0012.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0013.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0014.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DDX59-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72229-img0016.jpg "Immunohistochemistry: DDX59 Antibody (OTI2G1) - Azide and BSA Free [NBP2-72229] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")