DAPI Solution

Images

 
Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha ...read more
Immunohistochemistry: DAPI Solution [NBP2-31156] - DAPI labelling in mouse brain. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

DAPI Solution Summary

Description
DAPI is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. Dead cells which take up DAPI will fluoresce brightly around 461nm. DAPI may be excited by the UV or violet lasers although the UV laser is much more efficient. DAPI's emission spectra is very similar to the Pacific Blue (TM) dye.

Supplied at a concentration of 1 mg/ml.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 0.1-1.0ug/ml
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Reviewed Applications
Read 1 Review rated 4
using
NBP2-31156 in the following applications:

Publications
Read Publications using
NBP2-31156 in the following applications:

Reactivity Notes

Use in Human reported in scientific literature (PMID:33296752)

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
Water
Preservative
No Preservative
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for DAPI Solution

  • 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
  • 4,6-Diamidino-2-phenylindole dihydrochloride
  • DAPI dihydrochloride

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for DAPI Solution (NBP2-31156)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 8 applications: FLOW, ICC/IF, IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, IHC-P.


Filter By Application
FLOW
(3)
ICC/IF
(9)
IF
(1)
IF/IHC
(2)
IHC
(1)
IHC-Fr
(1)
IHC-FrFl
(1)
IHC-P
(4)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NBP2-31156 Applications Species
Buse J Einfluss einer ERK1/2-Resistenz auf Zellwachstum und Invasivit�t von triple-negativen Mammakarzinomzellen Thesis 2023-01-27
Juras J, Webb M, Young L et al. In situ microwave fixation provides an instantaneous snapshot of the brain metabolome Cell Reports Methods 2023-04-01 [PMID: 37159672] (IHC-FrFl, Mouse) IHC-FrFl Mouse
Ruckriegl S, Loris J, Wert K et al. Knockdown of G Protein-coupled Estrogen Receptor 1 (GPER1) Enhances Tumor-supportive Properties in Cervical Carcinoma Cells Cancer genomics & proteomics 2023-04-24 [PMID: 37093686] (ICC/IF, Human)

Details:
Dilutions: 1:1000
ICC/IF Human
Juras JA PERIPHERAL AND CENTRAL GLUCOSE FLUX IN TYPE I DIABETES Thesis 2022-01-01 (IHC-Fr) IHC-Fr
Boussaty E, Ninoyu Y, Andrade L et al. Altered Fhod3 Expression Involved in Progressive High-Frequency Hearing Loss via Dysregulation of Actin Polymerization Stoichiometry in The Cuticular Plate bioRxiv 2023-07-25 [PMID: 37546952]
Conroy LR, Clarke HA, Allison DB et al. Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis Nature communications 2023-05-13 [PMID: 37179348] (IHC-P) IHC-P
Berard AR, Brubaker DK, Birse K et al. Vaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling Cell reports 2023-05-05 [PMID: 37149863] (ICC/IF) ICC/IF
Sue YY Biological Correlates of Neuroimaging and a Systems Neuroimaging Approach to Examining Gene Environment Interactions in Psychiatric Disease Thesis 2021-01-01 (IF/IHC) IF/IHC
Saranya M, da Silva AM, Karjalainen H et al. Magnetic-Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering Advanced healthcare materials 2023-09-02 [PMID: 37660271] (IHC) IHC
Dervas E, Hepojoki J, Smura T, Prahauser B Python nidoviruses, more than respiratory pathogens bioRxiv 2020-01-01 (IHC-P) IHC-P
Show All 24 Publications.

Review for DAPI Solution (NBP2-31156) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-31156:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry DAPI Solution NBP2-31156
Enlarge
4
reviewed by:
Arun Satelli
ICC Human 10/09/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman Cancer cell line LM7
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for DAPI Solution (NBP2-31156). (Showing 1 - 1 of 1 FAQs).

  1. What is the concentration of 1mL of  DAPI Solution?
    • The concentration of this solution is 1 mg/ml. 

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Recent Reviews

4
5
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4
1
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1
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Arun Satelli
10/09/2014
Application: ICC
Species: Human