DAPI Solution Summary
DAPI is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. Dead cells which take up DAPI will fluoresce brightly around 461nm. DAPI may be excited by the UV or violet lasers although the UV laser is much more efficient. DAPI's emission spectra is very similar to the Pacific Blue (TM) dye.
Flow Cytometry Immunocytochemistry/Immunofluorescence 0.1-1.0ug/ml Immunohistochemistry Free-Floating Immunohistochemistry Whole-Mount Immunohistochemistry Immunohistochemistry-Frozen Immunohistochemistry-Paraffin
Use in Human reported in scientific literature (PMID:33296752)
Packaging, Storage & Formulations
Store at -20C in the dark. Avoid freeze-thaw cycles.
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Alternate Names for DAPI Solution
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride 4,6-Diamidino-2-phenylindole dihydrochloride DAPI dihydrochloride Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
for 6 months from date of receipt.
Publications for DAPI Solution (NBP2-31156)(16)
We have publications tested in 2 confirmed species: Human, Porcine.
We have publications tested in 6 applications: FLOW, ICC/IF, IF, IF/IHC, IHC-Fr, IHC-P.
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Publications using NBP2-31156
Buse J Einfluss einer ERK1/2-Resistenz auf Zellwachstum und Invasivit�t von triple-negativen Mammakarzinomzellen Thesis 2023-01-27
Su X, Gu X, Zhang Z et al. Retinoic acid receptor gamma is targeted by microRNA-124 and inhibits neurite outgrowth Neuropharmacology 2019-06-03 [PMID: 31170403] (IF) IF
Farombi EO, Awogbindin IO, Farombi TH et al. NEUROPROTECTIVE ROLE OF KOLAVIRON IN STRIATAL REDO-INFLAMMATION ASSOCIATED WITH ROTENONE MODEL OF PARKINSON'S DISEASE Neurotoxicology 2019-03-28 [PMID: 30930291] (ICC/IF) ICC/IF
Yi SY, Barnett BR, Torres-Velazquez M et al. Detecting Microglial Density With Quantitative Multi-Compartment Diffusion MRI Front Neurosci 2019-02-19 [PMID: 30837826] (ICC/IF) ICC/IF
Gupta K, Levinsohn J, Linderman G et al. Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis Dev. Cell 2018-12-19 [PMID: 30595533] (IHC-P) IHC-P
Ferreira F, Raghunathan V, Luxardi G et al. Early redox activities modulate Xenopus tail regeneration. Nat Commun. 2018-10-16 [PMID: 30327466] (ICC/IF) ICC/IF
Stenhouse C, Hogg CO, Ashworth CJ. Associations between fetal size, sex and placental angiogenesis in the pig Biol. Reprod. 2019-01-01 [PMID: 30137229] (ICC/IF, Porcine) ICC/IF
Hansjosten I, Rapp J, Reiner L et al. Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines Arch. Toxicol. 2017-11-08 [PMID: 29119250] (FLOW) FLOW
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC, Human)
Dervas E, Hepojoki J, Smura T, Prahauser B Python nidoviruses, more than respiratory pathogens bioRxiv 2020-01-01 (IHC-P)
Jung Y, Cackowski FC, Yumoto K et al. Abscisic acid regulates dormancy of prostate cancer disseminated tumor cells in the bone marrow Neoplasia (New York, N.Y.) 2021-01-01 [PMID: 33296752] (FLOW, Human) FLOW
Hellinger JW, SchOmel F, Buse JV et al. Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling Sci Rep 2020-10-21 [PMID: 33087801] (ICC/IF, Human) ICC/IF
Sue YY Biological Correlates of Neuroimaging and a Systems Neuroimaging Approach to Examining Gene Environment Interactions in Psychiatric Disease Thesis 2021-01-01 (IF/IHC)
Juras JA PERIPHERAL AND CENTRAL GLUCOSE FLUX IN TYPE I DIABETES Thesis 2022-01-01 (IHC-Fr)
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P) Details: Citation using the HRP version of this antibody. IHC-P
Chousal JN, Sohni A, Vitting-Seerup K et al. Progression of the pluripotent epiblast depends upon the NMD Factor UPF2 Development (Cambridge, England) 2022-10-18 [PMID: 36255229] (ICC/IF) Details: Dilution used in ICC 1:1000 ICC/IF
Show All 16 Publications.
DAPI Solution (NBP2-31156) (1)
Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
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Reviews using NBP2-31156:
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Verified Customer ICC
Application Immunocytochemistry Sample Tested Human Cancer cell line LM7 Species Human
Product General Protocols
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FAQs for DAPI Solution (NBP2-31156). (Showing 1 -
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What is the concentration of 1mL of DAPI Solution?
The concentration of this solution is 1 mg/ml.
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