DAPI Solution

Images

 
Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified ...read more
Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell ...read more

Product Details

Summary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF, IHC-FrFl, IHC-WhMt
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

DAPI Solution Summary

Description
DAPI is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. Dead cells which take up DAPI will fluoresce brightly around 461nm. DAPI may be excited by the UV or violet lasers although the UV laser is much more efficient. DAPI's emission spectra is very similar to the Pacific Blue (TM) dye.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.1-1.0ug/ml
Reviewed Applications
Read 1 Review rated 4
using
NBP2-31156 in the following application:

Publications
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NBP2-31156 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
Water
Preservative
No Preservative
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for DAPI Solution

  • 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
  • 4,6-Diamidino-2-phenylindole dihydrochloride
  • DAPI dihydrochloride

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for DAPI Solution (NBP2-31156)(6)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 3 applications: Flow, ICC/IF, IHC-P.


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ICC/IF
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Review for DAPI Solution (NBP2-31156) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-31156:
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ICC
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Images Ratings Applications Species Date Details
Immunocytochemistry DAPI Solution NBP2-31156
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reviewed by:
Arun Satelli
ICC Human 10/09/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman Cancer cell line LM7
SpeciesHuman
CommentsDAPI is used for fluoroscence imaging of cell nuclei in this case. At higher concentration 1:1000, the cell nuclei were very bright. However it is not as bright as DRAQ5, but given the wavelength difference, DAPI detection at UV range will suffice the need.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:3000

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsFixing using 4% PFA, Block in 0.25% FBS,
Wash Description5 min each 3 times in PBS

Comments

CommentsDAPI is used for fluoroscence imaging of cell nuclei in this case. At higher concentration 1:1000, the cell nuclei were very bright. However it is not as bright as DRAQ5, but given the wavelength difference, DAPI detection at UV range will suffice the need.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for DAPI Solution (NBP2-31156). (Showing 1 - 1 of 1 FAQs).

  1. What is the concentration of 1mL of  DAPI Solution?
    • The concentration of this solution is 1 mg/ml. 

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Contact Information

Product PDFs

Recent Reviews

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Arun Satelli
10/09/2014
Application: ICC
Species: Human