CyTRAK Orange™

Images

 
Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127] - CyTRAK Orange was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. ...read more
Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127] - Nucleated cell gating by CyTRAK Orange (TM )intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.
CyTRAK Orange (TM) [NBP2-81127] - Excitation and Emission Spectra for CyTRAK Orange (TM)
Fluorescence Imaging: CyTRAK Orange (TM) [NBP2-81127] - counterstaining of fixed U2OS cells, showing differential nuclear and cytoplasmic staining.
CyTRAK Orange (TM) [NBP2-81127] - Absorbance profile of CyTRAK Orange (TM)compared to eGFP and APC.
CyTRAK Orange (TM) [NBP2-81127] - Emission profile of CyTRAK Orange (TM)compared to eGFP and Cy7

Product Details

Summary
Applications Flow, ICC/IF, LIM, FI

Order Details

CyTRAK Orange™ Summary

Description
Key Features of CyTRAK Orange (TM):
  • Orange fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations
  • Rapid staining and easy to use, without a wash or lyse step
  • This dual compartment label provides quick, single cell feature discrimination such as cell location, cell perimeter, cell shape and cell spread parameters

  • CyTRAK Orange (TM) co-excites at 488 nm with eGFP and FITC labels avoiding the need for laser alignment in confocal instruments when using this combination of probes. Since CyTRAK Orange (TM) is not excited at 633 or 647 nm, a separate series of parameters can be detected with red-exciting fluorophores such as APC, APC-Cy7.
    Purity
    >97%

    Applications/Dilutions

    Dilutions
    • Flow Cytometry 1:500 - 1:1000
    • Immunocytochemistry/Immunofluorescence 1:1000
    • Fluorescence Imaging 1:1000
    • Live Imaging Microscopy 1:1000
    Application Notes
    CyTRAK Orange (TM) is supplied as a cranberry red aqueous solution at a concentration of 5mM: the 50 ul size covers 250 Imaging assays and 100 Flow Cytometry assays. The 200 ul size covers 1,000 Imaging assays, 6,600 HCS assays, and 400 Flow Cytometry assays.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark. Do not freeze.
    Purity
    >97%

    Notes

    Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. CyTRAK Orange (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution

    Background

    CyTRAK Orange (TM) is an orange fluorescent dye that stains the nucleus and cytoplasm but with different intensities. It functions as a DNA intercalating probe and does not crosslink with or esterify cytoplasmic proteins. Since nuclear staining is stronger than cytoplasmic staining, both cellular compartments can be easily identified during high content imaging screening. In addition CyTRAK Orange (TM) preferentially stains arrested / senescent cells compared to healthy cells, thus enabling easy identification and separation of these two distinct cellular populations.

    The excitation wavelength maximum of CyTRAK Orange (TM) is 510 nm and its emission wavelength maximum is 610 nm. CyTRAK Orange (TM) is not excited by the UV or red laser.

    CyTRAK Orange (TM) is a registered trademark of BioStatus Limited.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

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