Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
SPM591
Clonality
Monoclonal
Host
Mouse

Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) [Alexa Fluor® 405] Summary

Immunogen
Solubilized keratin extract from human stratum corneum
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 405
Format
Alexa Fluor 405 (A=405, E=421)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin, Basic (Type II or HMW) Antibody (NBP2-34435AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin, Basic (Type II or HMW) Products

Cytokeratin, Basic (Type II or HMW) NBP2-34435AF405

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