Cysteinyl Leukotriene ELISA Kit (Colorimetric)

ELISA: Cysteinyl Leukotriene ELISA Kit (Colorimetric) [NBP2-61288] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: Culture supernatants and urine from any species
• Standard Curve Range: 78.1 - 2,500 pg/ml
• Sensitivity: 26.6 pg/ml

Cysteinyl Leukotriene ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 78.1 - 2,500 pg/ml
• Sensitivity: 26.6 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Mean 6.4%
• Intra-Assay: Mean 4.6%
• Spike Recovery: Human Urine - 107.6%
  Tissue Culture Media - 101.0%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Background

Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Cysteinyl Leukotriene ELISA Kit (NBP2-61288). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.