Cysteinyl Leukotriene ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
78.1 - 2,500 pg/ml |
| Sensitivity |
26.6 pg/ml |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
Mean 6.4% |
| Intra-Assay |
Mean 4.6% |
| Spike Recovery |
Human Urine - 107.6% Tissue Culture Media - 101.0% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Antibody
- Assay buffer concentrate
- Conjugate
- GxM Microtiter plate
- pNpp Substrate
- Standard
- Stop solution
- Wash buffer concentrate
|
Background
Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Cysteinyl Leukotriene ELISA Kit (NBP2-61288). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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