Cysteinyl Leukotriene ELISA Kit (Colorimetric)

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Western Blot: Cysteinyl Leukotriene ELISA Kit (Colorimetric) [NBP2-61288] - Detection of human TMX4 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis ...read more
ELISA: Cysteinyl Leukotriene ELISA Kit (Colorimetric) [NBP2-61288] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Applications WB, ELISA
Suitable Sample Type
Culture supernatants and urine from any species.
Standard Curve Range
78.1 - 2,500 pg/ml
Sensitivity
26.6 pg/ml

Order Details

Cysteinyl Leukotriene ELISA Kit (Colorimetric) Summary

Standard Curve Range
78.1 - 2,500 pg/ml
Sensitivity
26.6 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
Mean 6.4%
Intra-Assay
Mean 4.6%
Spike Recovery
Human Urine - 107.6%
Tissue Culture Media - 101.0%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Antibody
  2. Assay buffer concentrate
  3. Conjugate
  4. GxM Microtiter plate
  5. pNpp Substrate
  6. Standard
  7. Stop solution
  8. Wash buffer concentrate

Background

Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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