Cysteine S-Nitrosylated Antibody (SNO-Cys)

Product Discontinued

Cysteine S-Nitrosylated Antibody (SNO-Cys) Summary

• Immunogen: Nitrosylated-Cystein, conjugated to KLH (hemocyanin).
• Specificity: Recognizes nitrosylated-cysteine modified proteins from all species. No significant reactivity is observed with unmodified BSA or Cysteine-BSA (not nitrosylated).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 0.1% BSA
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Background

Nitric oxide (NO), generated by cell type-specific NO-synthase (NOS) isoforms, is a freely diffusible intercellular messenger that functions in target cells in NOS-dependent signaling. S-nitrosylation of cysteine thiols in proteins by the highly labile NO radical has been identified as an important effector of NO-related bioactivity both in NOS-containing cells and intercellular signaling. Most cells contain low levels of nitrosylated proteins that are thought to be regulated by S-nitrosylation and denitrosylation. S-nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S-nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif that may also be subject to oxygen- or glutathione-dependent modification. NO-sensitive ion channels including the cardiac and skeletal muscle ryanodine receptor (RyR1), N-methyl-D-aspartate receptor (NMDAR) complex, cyclic-nucleotide gated ion channel, are modulated by S-nitrosylation. S-nitrosylation of capsase-3 inhibits apoptosis signaling. S-nitrosylation activates matrix metalloproteinase-9 (MMP-9) and induces neuronal apoptosis. The small G-protein p21Ras and Jun kinase are regulated by S-nitrosylation. The activity of transcription factors such as NFkB, c-jun, and c-fos is modulated by S-nitrosylation. In addition, the formation of S-nitrosylated glutathione (GSNO) has been proposed to be one of the major storage forms of NO in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.