cyclic GMP ELISA Kit (Colorimetric) Summary
| Description |
cyclic GMP Complete ELISA Kit is a kit for the quantitative determination of cyclic GMP in cells and tissue treated with 0.1N HCl, in addition to culture supernates, saliva, and serum. |
| Standard Curve Range |
Non-Acetylated format: 0.8 to 500 pmol/mL; Acetylated format: 0.08 to 50 pmol/mL |
| Sensitivity |
Assay Buffer: 0.42 pmol/mL (non-acetylated); 0.043 pmol/mL (acetylated) HCl: 0.604 pmol/mL (non-acetylated); 0.059 pmol/mL (acetylated) |
| Assay Type |
Quantification |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- 0.1 M HCl
- Acetic Anhydride
- Assay Buffer 2
- cGMP Antibody
- cGMP Conjugate
- cyclic GMP Standard
- Goat anti-Rabbit IgG Microtiter Plate
- Neutralizing Reagent
- Plate Sealer
- pNpp Substrate
- Stop Solution
- Triethylamine
- Wash Buffer Concentrate
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for cyclic GMP ELISA Kit (Colorimetric)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for cyclic GMP ELISA Kit (KA0321). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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