Human Cyclic Citrullinated Peptide Autoantibody ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum

Human Cyclic Citrullinated Peptide Autoantibody ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of human Cyclic Citrullinated Peptide Autoantibody. No significant cross-reactivity or interference between human Cyclic Citrullinated Peptide Autoantibody and analogues was observed.
• Assay Type: Indirect ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human Cyclic Citrullinated Peptide Autoantibody ELISA Kit (Colorimetric)

• Anti-CCP Antibody ELISA
• Anti-CCP ELISA
• CCP Antibody ELISA

Background

This assay employs the quantitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for Cyclic Citrullinated Peptide present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human Cyclic Citrullinated Peptide Autoantibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.