Cyclic AMP ELISA Kit (Colorimetric)

ELISA: Cyclic AMP ELISA Kit [Alkaline Phosphatase] [KA0320] - Standard curves

• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Cell Lysate, Saliva, Serum, Tissue
• Standard Curve Range: 0.078 to 20 pmol/mL
• Sensitivity: Assay Buffer: 0.30 pmol/mL (non-acetylated); 0.039 pmol/mL (acetylated)
  HCl: 0.39 pmol/mL (non-acetylated); 0.037 pmol/mL (acetylated)

Cyclic AMP ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. cyclic AMP Complete ELISA Kit is a kit for the quantitative determination of cyclic AMP in cells and tissue treated with 0.1M HCl, in addition to culture supernates, saliva, and serum.
• Standard Curve Range: 0.078 to 20 pmol/mL
• Sensitivity: Assay Buffer: 0.30 pmol/mL (non-acetylated); 0.039 pmol/mL (acetylated)
  HCl: 0.39 pmol/mL (non-acetylated); 0.037 pmol/mL (acetylated)
• Assay Type: Quantification
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cyclic AMP ELISA Kit (Colorimetric)

• 3' 5' cyclic adenosine monophosphate
• Cyclic adenosine monophosphate
• Cyclic AMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Cyclic AMP ELISA Kit (KA0320)(4)

Publications using KA0320

• Bianca C, Mathieu D, Fabio M et al. Atypical CXCL12 signaling enhances neutrophil migration by modulating nuclear deformability. Sci Signal. 2022-11-22 [PMID: 36413598]
• Camilla B, Matteo A, Tatiana V et al. The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites. Nat Metab. 2021-12-06 [PMID: 34873337]
• Francesca G, Martina S, Carlotta P et al. Cholesterol Activates Cyclic AMP Signaling in Metaplastic Acinar Cells. Metabolites. 2021-02-26 [PMID: 33652890]
• Stroth N, Niso M, Colabufo NA et al. Arylpiperazine Agonists of the Serotonin 5-HT1A Receptor Preferentially Activate cAMP Signaling versus Recruitment of beta-Arrestin-2. BMC. 2015-08-01 [PMID: 26081758]

FAQs for Cyclic AMP ELISA Kit (KA0320). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.