Mouse CTX-1 ELISA Kit (Colorimetric)

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Sample reference curve
ELISA: Mouse CTX-1 ELISA Kit (Colorimetric) [NBP3-11802] - Samples were spiked with high concentrations of Mouse CTX-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse CTX-1 ELISA Kit (Colorimetric) [NBP3-11802] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Mouse CTX-1 ELISA Kit (Colorimetric) Summary

Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.73
Intra-Assay
%CV < 5.45
Spike Recovery
86 - 108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Antibody Diluent
  2. Concentrated Biotinylated Detection Antibody
  3. Concentrated HRP Conjugate
  4. Concentrated Wash Buffer
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate
  7. Plate Sealer
  8. Reference Standard & Sample Diluent
  9. Reference Standard
  10. Stop Solution
  11. Substrate Reagent

Alternate Names for Mouse CTX-1 ELISA Kit (Colorimetric)

  • Collagen Type 1 C-Telopeptide
  • Cross Linked C-telopeptide of Type I Collagen
  • CTX
  • CTXI

Background

CTX-1, also referred to as either carboxy-terminal cross-linked telopeptide of type 1 collagen or C-terminal end of the telopeptide of type I collagen, is a marker of osteoporosis and bone resorption (1,2). CTX-1 is a common bone turnover marker (BTM) that can easily be measured in the plasma, serum, or urine (1-3). Bone turnover refers to the removal of old bones by resorption followed by generation of new bones (3). CTX-1 is released from intact collagen type 1 by cathepsin K cleavage and can be detected by an antibody against the eight amino acid sequence EKAHDGGR (2,4). Levels of CTX-1 show circadian rhythm variabilities altered by food intake, which makes timing of sample collection an important consideration and is typically done in a fasted state (1,3). Studies show that increased levels of CTX-1 along with bone-specific alkaline phosphatase (BSALP) is associated with increased risk for bone fractures and osteoporosis (3). The most common treatment for osteoporosis is bisphosphonates, which are classified as anti-resorptive drugs that slow the removal of bone (1,3). Another treatment is the Denosumab, a monoclonal antibody for receptor activator of NFkappaB ligand, which shows decreased levels of CTX-1 and other markers within the first 24-hours of treatment (1-3). Conversely, anabolic therapies add bone by focusing on the osteoclast and osteoblast populations. Romosozumab is one specific anabolic drug that showed quick decrease in CTX-1 levels following treatment (2,3).

References

1. Williams, C., & Sapra, A. (2020). Osteoporosis Markers. In StatPearls. StatPearls Publishing.

2. Szulc P. (2018). Bone turnover: Biology and assessment tools. Best Practice & Research. Clinical Endocrinology & Metabolism. https://doi.org/10.1016/j.beem.2018.05.003

3. Park, S. Y., Ahn, S. H., Yoo, J. I., Chung, Y. J., Jeon, Y. K., Yoon, B. H., Kim, H. Y., Lee, S. H., Lee, J., & Hong, S. (2019). Position Statement on the Use of Bone Turnover Markers for Osteoporosis Treatment. Journal of Bone Metabolism. https://doi.org/10.11005/jbm.2019.26.4.213

4. Cremers, C., Garnero, P., Seibel, M. J. (2008). Chapter 87- Biochemical Markers of Bone Metabolism. Principles of Bone Biology (Third Edition). https://doi.org/10.1016/B978-0-12-373884-4.00020-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Additional CTX-1 Products

Array NBP3-11802

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