![Dot Blot: Crotonaldehyde Antibody (2D3.1) [NBP2-61922] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2D3.1 (NBP2-61922). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61922) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.](http://images.novusbio.com/fullsize/Crotonaldehyde-Antibody-2D3-1-Dot-Blot-NBP2-61922-img0002.jpg "Dot Blot: Crotonaldehyde Antibody (2D3.1) [NBP2-61922] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2D3.1 (NBP2-61922). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61922) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, DB, ELISA |
| Clone | 2D3.1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Specificity | Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | A 1:1000 dilution of this antibody was sufficient for detection of Crotonaldehyde-modified proteins in 1 ug of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")