![Dot Blot: Crotonaldehyde Antibody (2A8.1) [NBP2-61918] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2A8.1 (NBP2-61918). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61918) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.](http://images.novusbio.com/fullsize/Crotonaldehyde-Antibody-2A8-1-Dot-Blot-NBP2-61918-img0002.jpg "Dot Blot: Crotonaldehyde Antibody (2A8.1) [NBP2-61918] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2A8.1 (NBP2-61918). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61918) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.")
| Immunogen | Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Specificity | Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | A 1:1000 dilution of this antibody was sufficient for detection of Crotonaldehyde-modified proteins in 1 ug of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibod |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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