CpG oligodeoxynucleotides

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CpG oligodeoxynucleotides [NBP2-26238]
CpG oligodeoxynucleotides [NBP2-26238] - Evaluation of various CpG ODN effects on activation of human TLR9. TLR9/NF-kB/SEAPorter HEK 293 cells were plated in 96-well plates at 7.5 x 10^4 cells/well for 16 h. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications LA
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

CpG oligodeoxynucleotides Summary

Specificity
CpG ODNs, TLR9 Ligands (human)

Applications/Dilutions

Dilutions
  • Ligand Activation 5-20 ug/ml
Application Notes
Human CpG ODNs are used for activation of human TLR9 at a range of 5-20 ug/ml. The TLR9/NF-kB/SEAPorter HEK 293 cell line (IML-109) is a useful positive control model system for studying hTLR9 activation (Figure 1). We typically use a 96-well plate format assay using this reporter cell line
Publications
Read Publication using NBP2-26238.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile water
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Kit Components

Components
  1. NBP2-31139SS: 25 ug CpG Type C ODN (C792)
  2. NBP2-31138SS: 25 ug CpG Type C ODN (C695)
  3. NBP2-31137SS: 25 ug CpG Type C ODN (C274)
  4. NBP2-31136SS: 25 ug CpG Type A ODN (2216)
  5. NBP2-31134SS: 25 ug CpG Type B ODN (2006)

Alternate Names for CpG oligodeoxynucleotides

  • CpG ODN (2006)
  • CpG ODN
  • oligodeoxynucleotides (ODN)

Background

Unmethylated deoxycytosine-deoxyguanosine (CpG) dinucleotides are more frequently present in bacterial and viral DNA than in mammalian DNA1. CpG oligodeoxynucleotides (ODNs) are synthetic forms of oligonucleotides, which contains unmethylated CpG motifs, and can induce cellular immune responses through the activation of Toll-like receptor 9 (TLR9)2,3. There are three types (types A, B and C) of CpG ODNs, each of which acts differentially on stimulation of immune cells3-5. Type A CpG ODNs consist of a phosphodiester central CpG-containing palindromic motif and a phosphorothioate poly-G string at 3'-end. Type A CpG ODNs are high inducers for IFNa production in plasmacytoid dendritic cells (pDCs) but are low inducers for TLR9-dependent NF-kB induction. Type B CpG ODNs consist of a full phosphorothioate backbone with one or more CpG motifs. They are relatively higher inducers for TLR9-dependent NF-kB induction, strongly activating B cells, but are low inducers for IFNa production in pDCs. Type C CpG ODNs contain both features of Types A and B, which consist of a full phosphorothioate backbone and a CpG-containing palindromic motif. Type C CpG ODNs can induce high IFNa production in pDCs as well as B cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for CpG oligodeoxynucleotides (NBP2-26238)(1)

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