CpG oligodeoxynucleotides Mouse

CpG oligodeoxynucleotides Mouse [NBP2-26240]

CpG oligodeoxynucleotides Mouse [NBP2-26240] - Evaluation of various CpG ODN effects on activation of mouse TLR9. NF-kB/SEAPorter RAW cells were plated in 96-well plates at 7.5 x 10^4 cells/well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit.

• Reactivity: Mu
• Applications: Func
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

CpG oligodeoxynucleotides Mouse Summary

• Description:

  Mouse Sequence CpG ODN (1585) Type A: 5' G*GGGTCAACGTTGAG*G*G*G*G*G 3'

  Mouse Sequence CpG ODN (1826) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*C*G*T*T 3'

  Mouse Sequence CpG ODN(1668) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*T*G*C*T 3'

  Mouse Sequence CpG ODN (1018) Type B: 5' T*G*A*C*T*G*T*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A 3'

  Human/Mouse Sequence CpG ODN (C274) Type C: 5' T*C*G*T*C*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A*T 3'

  Human/Mouse Sequence CpG ODN (C695) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T 3'

  Human/Mouse Sequence CpG ODN (C792) Type C: 5'T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*T 3'
  
  (* Indicates a phosphorothioate modification)

  Functionality:This product is useful for the activation of TLR9 and the stimulation of TLR9 has been reported with 5-20 ug/ml.

• Specificity: CpG oligodeoxynucleotides Mouse, TLR9 Ligands (mouse)

Applications/Dilutions

• Dilutions:
  • Ligand Activation 5 -20 ug/ml
• Application Notes: A TLR9/NF-kB SEAP reporter construct in a HEK 293 cell line was used as a model system for studying hTLR9 activation.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile water
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for CpG oligodeoxynucleotides Mouse

• CpG oligodeoxynucleotides (ODN)
• ODN

Background

Unmethylated deoxycytosine-deoxyguanosine (CpG) dinucleotides are more frequently present in bacterial and viral DNA than in mammalian DNA1. CpG oligodeoxynucleotides (ODNs) are synthetic forms of oligonucleotides, which contains unmethylated CpG motifs, and can induce cellular immune responses through the activation of Toll-like receptor 9 (TLR9)2,3. There are three types (types A, B and C) of CpG ODNs, each of which acts differentially on stimulation of immune cells3-5. Type A CpG ODNs consist of a phosphodiester central CpG-containing palindromic motif and a phosphorothioate poly-G string at 3'-end. Type A CpG ODNs are high inducers for IFNa production in plasmacytoid dendritic cells (pDCs) but are low inducers for TLR9-dependent NF-kB induction. Type B CpG ODNs consist of a full phosphorothioate backbone with one or more CpG motifs. They are relatively higher inducers for TLR9-dependent NF-kB induction, strongly activating B cells, but are low inducers for IFNa production in pDCs. Type C CpG ODNs contain both features of Types A and B, which consist of a full phosphorothioate backbone and a CpG-containing palindromic motif. Type C CpG ODNs can induce high IFNa production in pDCs as well as B cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for CpG oligodeoxynucleotides Mouse (NBP2-26240)(1)

Publication using NBP2-26240

• Gillaux C, Mehats C, Vaiman D et al. Functional screening of TLRs in human amniotic epithelial cells. J Immunol. 2011-09-01 [PMID: 21775685]
  
  Details:
  TLR ligands: TLR1/2 (IMG-2201), TLR3 (IMG-2203), TLR4 (IMG-2204), TLR5 (IMG-2205), TLR6/2 (IMG-2206), TLR7 (IMG-2207), TLR9 (IMG-2209Hpt). The effects of ligand stimulation was measured by various readout assays, refer to the figures for details (Figs 2-8, S1).