CpG oligodeoxynucleotides Mouse

Images

 
CpG oligodeoxynucleotides Mouse [NBP2-26240]
CpG oligodeoxynucleotides Mouse [NBP2-26240] - Evaluation of various CpG ODN effects on activation of mouse TLR9. NF-kB/SEAPorter RAW cells were plated in 96-well plates at 7.5 x 10^4 cells/well for 16 h. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

CpG oligodeoxynucleotides Mouse Summary

Specificity
CpG oligodeoxynucleotides Mouse, TLR9 Ligands (mouse)

Applications/Dilutions

Application Notes
Mouse CpG ODNs are used for activation of mouse TLR9 at a range of 5-20 ug/ml. The NF-kB/SEAPorter RAW cell line is a useful positive control model system for studying mTLR9. We typically use a 96-well plate format assay using this reporter cell line
Publications
Read Publication using NBP2-26240.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile water
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Kit Components

Components
  1. NBP2-31139SS: 25 ug CpG Type C ODN (C792)
  2. NBP2-31141SS: 25 ug CpG Type B ODN (1668)
  3. NBP2-31140SS: 25 ug CpG Type A ODN (1585)
  4. NBP2-31142SS: 25 ug CpG Type B ODN (1018)

Alternate Names for CpG oligodeoxynucleotides Mouse

  • CpG oligodeoxynucleotides (ODN)
  • ODN

Background

Unmethylated deoxycytosine-deoxyguanosine (CpG) dinucleotides are more frequently present in bacterial and viral DNA than in mammalian DNA1. CpG oligodeoxynucleotides (ODNs) are synthetic forms of oligonucleotides, which contains unmethylated CpG motifs, and can induce cellular immune responses through the activation of Toll-like receptor 9 (TLR9)2,3. There are three types (types A, B and C) of CpG ODNs, each of which acts differentially on stimulation of immune cells3-5. Type A CpG ODNs consist of a phosphodiester central CpG-containing palindromic motif and a phosphorothioate poly-G string at 3'-end. Type A CpG ODNs are high inducers for IFNa production in plasmacytoid dendritic cells (pDCs) but are low inducers for TLR9-dependent NF-kB induction. Type B CpG ODNs consist of a full phosphorothioate backbone with one or more CpG motifs. They are relatively higher inducers for TLR9-dependent NF-kB induction, strongly activating B cells, but are low inducers for IFNa production in pDCs. Type C CpG ODNs contain both features of Types A and B, which consist of a full phosphorothioate backbone and a CpG-containing palindromic motif. Type C CpG ODNs can induce high IFNa production in pDCs as well as B cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Supplier Logo

Publications for CpG oligodeoxynucleotides Mouse (NBP2-26240)(1)

Reviews for CpG oligodeoxynucleotides Mouse (NBP2-26240) (0)

There are no reviews for CpG oligodeoxynucleotides Mouse (NBP2-26240). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for CpG oligodeoxynucleotides Mouse (NBP2-26240): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CpG oligodeoxynucleotides Mouse (NBP2-26240) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional CpG oligodeoxynucleotides Mouse Products

Blogs on CpG oligodeoxynucleotides Mouse

There are no specific blogs for CpG oligodeoxynucleotides Mouse, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Contact Information

Product PDFs

Review this Product

Be the first to review our CpG oligodeoxynucleotides Mouse and receive a gift card or discount.